This letter presents an active coherent beam combining (CBC) of a pulsed laser based on sampling the intrapulse evaluation function. By precisely controlling trigger sequence of an analog-to-digital converter (ADC), fixed time point of the pulse light is sampled as evaluation function for CBC. The active CBC of two fiber amplifiers with a 500 ns pulse width and 10 kHz repetition rate is experimentally demonstrated by applying a hill climbing algorithm. The residual phase error is approximately λ/27. A coherent Doppler wind lidar (CDWL) based on CBC light source is verified.The experimental results verify the feasibility of using the pulsed CBC to improve the pulse energy of a CDWL without degrading performance.