Journals >Journal of Innovative Optical Health Sciences
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2025
Volume: 18 Issue 3
12 Article(s)
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Research Articles
Ultrabright quantum dots assisted in vivo NIR-II fluorescence microscopic imaging for brain metastases in triple-negative breast cancer
Yuxiang Gao, Chi Zhang, Lijun Zhu, Zhong Du, Rong Ma, Le Guo, Nuernisha Alifu, and Xueliang Zhang
Triple-negative breast cancer (TNBC) is an aggressive and often fatal disease, especially since the brain metastasis of TNBC has been a particularly severe manifestation. However, brain metastasis in TNBC at early stages often lacks noticeable symptoms, making it challenging to detect. Near-infrared II (NIR-II) fluoresTriple-negative breast cancer (TNBC) is an aggressive and often fatal disease, especially since the brain metastasis of TNBC has been a particularly severe manifestation. However, brain metastasis in TNBC at early stages often lacks noticeable symptoms, making it challenging to detect. Near-infrared II (NIR-II) fluorescence microscopic imaging obtains long wavelength, which enables reduced scattering, high spatial resolution and minimal autofluorescence, it is also a favorable imaging method for tumor diagnosis. PbS@CdS quantum dots (QDs) are one of the popular NIR-II fluorescence nanoprobes for well brightness. In this study, NIR-II emissive PbS@CdS QDs were utilized and further encapsulated with thiol-terminated poly(ethylene oxide) (SH-PEG, MW = 5000) to form PbS@CdS@PEG QDs nanoparticles (NPs). The obtained PbS@CdS@PEG QDs NPs were then characterized and further studied in detail. The PbS@CdS@PEG QDs NPs had large absorption spectra, exhibited strong NIR-II fluorescence emission at approximately 1300
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2441001 (2025)
Erbium-based hydrothermal YOF with stark sublevels for in vivo NIR II luminescence nanothermometer
Zhenghao Liu, Ziyue Ju, Hanyu Liu, Zhan Wang, and Ruichan Lv
Rare earth luminescent materials have attracted extensive attention in the biomedical field as noncontact temperature monitoring devices with microscopic resolution due to their properties in the visible and near-infrared regions. At the application level, it is required to have a certain temperature monitoring capabilRare earth luminescent materials have attracted extensive attention in the biomedical field as noncontact temperature monitoring devices with microscopic resolution due to their properties in the visible and near-infrared regions. At the application level, it is required to have a certain temperature monitoring capability in the near-infrared region II window to enhance the tissue penetration depth. Here, two kinds of YOFs: Er
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2441002 (2025)
Clickable HaloTag ligands for live cell labeling and imaging
Yunhe Luo, Siyu Zhou, Mingyue Gong, Changfeng Wu, and Xiaofeng Fang
Self-labeling protein (SLP) tags, such as HaloTag, have gained considerable interest as advanced tools for live cell labeling. However, the chloroalkane-based substrates that can be directly used for protein labeling are limited. Here, we report two bioorthogonal small molecule linkers, chloroalkane-tetrazine (CA-Tz) aSelf-labeling protein (SLP) tags, such as HaloTag, have gained considerable interest as advanced tools for live cell labeling. However, the chloroalkane-based substrates that can be directly used for protein labeling are limited. Here, we report two bioorthogonal small molecule linkers, chloroalkane-tetrazine (CA-Tz) and chloroalkane-azide (CA-N3), which can penetrate cell membranes and facilitate click chemistry-based labeling in live cells. We compare their labeling capability using two clickable silicon rhodamine dyes (SiR-PEG3-TCO and SiR-PEG4-DBCO). Confocal imaging results demonstrate that using CA-Tz and SiR-PEG3-TCO dye exhibits superior intracellular labeling with low nonspecific signals. We subsequently compared the photostability of SiR dyes with that of green fluorescent proteins (mEmerald). Total internal reflection fluorescence (TIRF) imaging indicates that SiR dyes exhibit superior photostability under identical excitation conditions, making them suitable for long-term cell imaging. Furthermore, SiR dyes labeling also shows high structure retention for the fourth-order super-resolution optical fluctuation imaging (SOFI) compared to fluorescent proteins. This study presents clickable HaloTag linkers as effective tools for live cell labeling and imaging, highlighting the high-quality labeling of chloroalkane linkers and clickable dyes for live cell imaging..
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2441003 (2025)
Introduction to Special Issue on Fluorescent Probes for Optical Imaging and Biosensing
Changfeng Wu, Chenguang Wang, and Wei Chen
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2502002 (2025)
Advances in fluorescent nanoprobes for live-cell super-resolution imaging
Peng Xu, Zexuan Dong, Simei Zhong, Yu-Hui Zhang, and Wei Shen
The rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with suThe rapid development of super-resolution microscopy has made it possible to observe subcellular structures and dynamic behaviors in living cells with nanoscale spatial resolution, greatly advancing progress in life sciences. As hardware technology continues to evolve, the availability of new fluorescent probes with superior performance is becoming increasingly important. In recent years, fluorescent nanoprobes (FNPs) have emerged as highly promising fluorescent probes for bioimaging due to their high brightness and excellent photostability. This paper focuses on the development and applications of FNPs as probes for live-cell super-resolution imaging. It provides an overview of different super-resolution methods, discusses the performance requirements for FNPs in these methods, and reviews the latest applications of FNPs in the super-resolution imaging of living cells. Finally, it addresses the challenges and future outlook in this field..
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2530001 (2025)
The second near-infrared (NIR-II) window excitable/emissive organic/polymeric fluorescent molecules for bioimaging application
Guannan Liu, Chenguang Wang, and Geyu Lu
The fluorescence imaging (FLI) in the second near-infrared window (NIR-II, 1000–1700nm) has attracted considerable attention in the past decade. In contrast to conventional NIR-I window excitation (808nm/980nm), FLI with NIR-II window excitation (1064nm/other wavelength beyond 1000nm) can afford deeper tissue penetratiThe fluorescence imaging (FLI) in the second near-infrared window (NIR-II, 1000–1700
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2530002 (2025)
Recent application of near-infrared fluorescence probes in food safety detection
Lei Li, Zhongrui Peng, Yun Zeng, and Gang Liu
Fluorescent probes have wide applications in biological and environmental analysis due to their advantages of simple operation, convenient flexibility, high sensitivity and efficiency. They are considered to be promising tools for accurate analysis of agriculture- and food-related hazardous substances. In this review, Fluorescent probes have wide applications in biological and environmental analysis due to their advantages of simple operation, convenient flexibility, high sensitivity and efficiency. They are considered to be promising tools for accurate analysis of agriculture- and food-related hazardous substances. In this review, the types and characteristics of the near-infrared fluorescence probes (NIFPs) are briefly described. The recent advances of NIFPs for precisely detecting various hazardous substances including heavy metals, sulfite and related sulfiting agents and hydrogen peroxide are summarized. Finally, the present challenges and future perspectives faced by NIFPs in food safety analysis are discussed..
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2530003 (2025)
Activatable fluorescent probes for imaging and diagnosis of hepatocellular carcinoma
Xiaoxiao Men, Fanghong Liu, Mingyue Gong, Xuchao Wang, Ling Wang, Xiaoju Men, Haobin Chen, and Zhuxin Dong
Liver cancer, specifically hepatocellular carcinoma (HCC), is a malignant neoplasm of the digestive system, characterized by exceptionally high morbidity and mortality rates on a global scale. Early detection and diagnosis are critical measures for enhancing the prognosis of patients diagnosed with HCC. An improved proLiver cancer, specifically hepatocellular carcinoma (HCC), is a malignant neoplasm of the digestive system, characterized by exceptionally high morbidity and mortality rates on a global scale. Early detection and diagnosis are critical measures for enhancing the prognosis of patients diagnosed with HCC. An improved prognosis is significantly reliant on the timely diagnosis of the disease and effective therapeutic monitoring. Activatable fluorescent probes are essential for detecting and imaging biomarkers related to disease diagnosis and in vivo imaging. This paper reviews the fluorescent probes developed over the past five years for the detection and imaging of HCC. This noninvasive optical imaging modality demonstrates significant promise in targeting pathological sites and is anticipated to facilitate potential clinical translation..
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2530004 (2025)
Recombinant PASylated nanobody probes with improved blood circulation and tumor targeting
Yicheng Yang, Lingyue Jin, You Zhang, Siyu Zhou, Weijun Wei, Gang Huang, and Changfeng Wu
Nanobodies have been extensively demonstrated in biomedical imaging and therapy. However, nanobody probes often suffer from rapid renal clearance due to its small size. Herein, we reported a recombinant nanobody with a 200 amino-acid polypeptide chain consisting of Pro, Ala, and Ser (PAS) at the C-terminal, which can bNanobodies have been extensively demonstrated in biomedical imaging and therapy. However, nanobody probes often suffer from rapid renal clearance due to its small size. Herein, we reported a recombinant nanobody with a 200 amino-acid polypeptide chain consisting of Pro, Ala, and Ser (PAS) at the C-terminal, which can be easily expressed in Escherichia coli with a high yield. The PASylated nanobody was functionalized with a fluorescent dye and the cell labeling properties were characterized by flow cytometry and confocal microscopy. In vivo fluorescence imaging indicated that the PASylated nanobody showed comparable blood circulation time, but
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2541001 (2025)
Engineering bright J-aggregates through manipulation of electron acceptor for in vivo NIR-II fluorescence imaging
Yonghui Pan, Xianwei Weng, Mingxuan Jia, Xiaofei Miao, Hui Zhao, Jie Zhang, Wenbo Hu, and Quli Fan
Fluorophores emitting in the second near-infrared window (NIR-II, 900–1700nm) allow for high-resolution deep-tissue bioimaging owing to minimal tissue scattering. Although J-aggregation offers a promising approach to developing long-wavelength emitters, the scarcity of J-type backbones and reliable design principles liFluorophores emitting in the second near-infrared window (NIR-II, 900–1700
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2541002 (2025)
Near-infrared Aza-BODIPY nanoparticles based on D-A-D structure for bioimaging
Haitao Liao, Qingxuan Meng, Yuhao Li, Senqiang Zhu, Rui Liu, and Hongjun Zhu
Optical imaging in vivo holds significant implications for disease diagnosis, and nanoprobes with near-infrared (NIR) emission leverage the deep tissue penetration and high spatiotemporal resolution provided by NIR light, demonstrating considerable application potential. This study presents the design and synthesis of Optical imaging in vivo holds significant implications for disease diagnosis, and nanoprobes with near-infrared (NIR) emission leverage the deep tissue penetration and high spatiotemporal resolution provided by NIR light, demonstrating considerable application potential. This study presents the design and synthesis of three nitrogen-doped boron–dipyrrin (Aza-BODIPY) molecules: Aza–BDP–OCH3, Aza–BDP–OH, and Aza–BDP-I. Leveraging the strong electron-accepting properties of the Aza-BODIPY core, we developed a donor–acceptor–donor (D-A-D) structure for Aza–BDP–OCH3 through modifications with triphenylamine and methoxy groups, resulting in NIR fluorescence. Aza–BDP–OH was obtained via demethylation using boron tribromide, whereas Aza–BDP-I was synthesized by introducing iodine into Aza–BDP–OCH3. These three molecules self-assemble with the amphiphilic polymer PMHC
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2541003 (2025)
Deep learning-enhanced NIR-II fluorescence volumetric microscopy for dynamic 3D vascular imaging
Shiyi Peng, Yuhuang Zhang, Xuanjie Mou, Tianxiang Wu, Mingxi Zhang, and Jun Qian
Three-dimensional (3D) visualization of dynamic biological processes in deep tissue remains challenging due to the trade-off between temporal resolution and imaging depth. Here, we present a novel near-infrared-II (NIR-II, 900–1880nm) fluorescence volumetric microscopic imaging method that combines an electrically tunaThree-dimensional (3D) visualization of dynamic biological processes in deep tissue remains challenging due to the trade-off between temporal resolution and imaging depth. Here, we present a novel near-infrared-II (NIR-II, 900–1880
Journal of Innovative Optical Health Sciences
- Publication Date: Jun. 18, 2025
- Vol. 18, Issue 3, 2550013 (2025)
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