
- Advanced Photonics
- Vol. 4, Issue 4, 046006 (2022)
Abstract
1 Introduction
Microscopes are important imaging tools that effectively expand the human eyes to the microworld for applications in scientific research, biomedical diagnosis, and industry. Besides the ultimate goal of superresolution, a new direction of this technique aims for wide field-of-view (FOV), large depth-of-field (DOF), high throughput, and compactly portable performance.1
A metalens, as a kind of metasurface composed of subwavelength units, opens a new avenue to access ultracompact and lightweight optical imaging systems.13,14 According to the powerful capability in manipulating the phase, polarization, and amplitude of light,15
In this work, we developed a polarizer-embedded metalens imaging device (PMID) that is implemented based on geometric silicon nitride () metasurfaces directly mounted on a CMOS image sensor. It is proved to significantly improve the imaging quality by eliminating the background noise, which enables zoomed-in imaging to reach a higher resolution. After a systematic characterization of the imaging performances from the singlet metalens, we extended it to a metalens array with two sets of polarization multiplexed focusing phases that work in co-and cross-polarizations, respectively. Based on this design, the embedded circular polarization filter (CPF) can work for both orthogonal polarization illuminations to construct two sets of compensated subimages, which enables a stitched high-quality large FOV microscope image. Thanks to the broadband property, this metalens array inherits the dispersive spectral zoom effect32,43 and shows the capability of large DOF imaging by tuning the illumination wavelength. A chip-scale () FOV is obtained with the resolution of ( in theory, limited by the size of the image sensor pixel), DOF of within a wavelength of 450 to 510 nm, which definitely exceeds the performance of traditional microscopes with the same resolution. Furthermore, it is fully implemented in a compact prototype with a light-emitting diode (LED) light source and a liquid-crystal polarization switch, which provides a non-mechanical, stable, and miniaturized microscope technique for portable and convenient applications.
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2 Device Architecture and Metalens Design
Figure 1(a) shows the layered architecture of the chip-scale PMID. The chip-scale metalens and fixed CPF with a thickness of are fixed on the CMOS image sensor (Imaging source: DMM 27UJ003-ML, pixel size: ) by two layers of optically clear adhesive tapes (OCA, thickness: and , Tesa, 69402). Here, the OCA works both as the connector and the spacer that determine the imaging distance (), which is fixed in our chip-scale PMID. A photographic image is depicted in Fig. 1(b).
Figure 1.Architecture and characterization of PMID. (a) Schematic of the chip-scale PMID. (b) Photographic image of the PMID with bottom-left inset for the side-view of the zoomed-in image, and upper-right inset for the SEM image of the
The phase profile of the metalens is designed according to the following hyperbolic distribution:13
3 Imaging Characterization of Singlet-Metalens PMID
Before getting to the large FOV imaging device, first, we characterized the imaging performances of a singlet-metalens PMID, including the resolution, signal-to-noise ratio (SNR), modulation transfer function (MTF), and imaging bandwidth. The experimental details are provided in Sec. S1 and Fig. S2 of the Supplementary Material. Note that we also measured a set of imaging performances with the same metalens imaging device but without the embedded CPF for comparison. Figures 1(c) and 1(d) show the images of a 1951 United States Air Force (USAF) resolution test chart taken through metalenses without/with CPF at different magnifications, respectively. Because CPF can filter out the background co-polarized light, the image quality (including SNR and resolution) with CPF is much better than that without CPF, especially under large magnification, as shown in Fig. 1(e). To quantitatively evaluate the quality of imaging, we defined , where and refer to the intensity of the signal that can be clearly resolved and the standard deviation of the background noise intensity, respectively. Next, we calculated SNR based on multiple sets of signals and noises for obtaining accurate SNR of two different conditions and reached average values of , 16.22/24.60, and 13.01/27.60 dB, respectively. The corresponding resolutions for three cases are (limited by CMOS pixel size: ), , and 870 nm at imaging distance , 1000, and , respectively, as shown in Fig. 1(g).
The MTFs of all metalenses are obtained by the slanted-edge method,45 as shown in Fig. 1(f), which show a good agreement with theoretical MTF at a low spatial frequency in all metalenses. The discrepancies at a high spatial frequency probably contribute to the background noises. The slanted-edge method is to calculate the Fourier transform of the line spread function, which is the first derivative of the edge spread function (ESF) (i.e., ). Although the PMID has a fixed imaging distance, optical zoom can be realized to obtain depth information by tuning the working wavelength due to the chromatic dispersion of the metalens. The details are provided in Sec. S3 of the Supplementary Material.
4 Dual-Phase Design for Co- and Cross-Polarization
In our previous work, a polarization multiplexed dual-phase metalens array was proposed and implemented in the CMOS integrated imaging device.36 It successfully expanded the FOV without increasing the working distance, and the intersecting dual-phase effectively compensated the blind areas in imaging stitching. However, the dual-phase design was based on two orthogonal polarizations that cannot work under a fixed CPF embedded in the integrated device. It led to a relatively poor imaging quality in imaging contrast and SNR, since the PB phase efficiency cannot reach the unity and results in inevitable background noises.
Figure 1(d) has shown the significant improvements in PMID imaging quality by adding the CPF compared with that without CPF [see Fig. 1(c)], and it is necessary to validate it in the polarization multiplexed lens array. To circumvent this problem, we proposed a new kind of polarization multiplexed metalens design that can modulate the co- and cross-polarized light independently by combining the propagation and PB phase. In a circle base, the Jones matrix of nanofin in a metasurface describing the relation between the input electric field () and the output electric field () can be written as27
Similarly, for the left-handed circularly polarized (LCP):
By adopting this design to our integrated scheme, two independent phase profiles can be obtained on output with a fixed polarization filter. For example, if the output light is set as RCP, then the incident RCP light can be designed for a special function with the phase profile and the incident LCP light with another phase profile . In that case, we can achieve a new polarization-switchable metasurface. The schematics of the electric field change of our design are shown in Fig. 2(a); the output light can be written as and under two orthogonal circularly polarized light beams, where and are the corresponding amplitudes of the output beams. For analyzing the amplitudes of the output light, focus on a single nanofin. When illuminated by a circularly polarized light, the transmitted light can be expressed as46
Figure 2.Independent dual-phase design for co- and cross-circularly polarized light. (a) Schematics of the design principle: a single metasurface obtains two independent phase modulations for each incident circular polarization light after fixed CPF (for RCP). Simulated phase responses of the transmitted (b) RCP and (c) LCP light with the nanofins structural parameters under RCP incidence. (d) Amplitude distribution of the selected nanofin structures, where red circles and blue squares correspond to co-polarized (b) and cross-polarized (c) phase modulations, respectively. The simulated intensity distributions of a transverse bifocal metalens on the focal plane with CPF for (e)
In the PMID, it is best to have to ensure the to get comparable intensities in two polarization states. The simulated phase responses of nanofins for the transmitted RCP and LCP light are shown in Figs. 2(b) and 2(c), respectively. The red circles and blue squares correspond to co-polarized and cross-polarized manipulations of the selected nanofins with the required phase responses. Figure 2(d) shows the amplitude distribution of the selected nanofins. By selecting a suitable size and orientation angle of nanofins, the phase manipulation is completely decoupled for two incident circularly polarized light beams. As a proof of concept, we simulated the response of a transverse bifocal metalens with a focal length of , radius of , and focal shift distance of by FDTD. The simulation intensity profiles on the focal plane with CPF for -polarized, LCP, and RCP light incidence are shown in Figs. 2(e)–2(g), respectively, and are exactly consistent with the design.
5 Wide-Field and Depth-of-Field Imaging by Chip-Scale PMID
Based on the co- and cross-polarization multiplexed metalens array, we proposed and implemented a chip-scale PMID in a system with an FOV of . The phase profiles of the metalens satisfy the requirements of two different focusing lenses for two orthogonal circularly polarized light beams, as schematically shown in Fig. 3(a) (more details about the design of a chip-scale metalens are provided in Sec. S4 of the Supplementary Material), where the lattices A and B correspond to the focusing phase distributions under LCP and RCP incidences, respectively. Each metalens is designed at 470 nm with focal lengths equal to and diameters of . Then, these metalenses compose a focusing array for LCP and ones for RCP incidence according to the dual-phase design. The entire metalens region is about , nearly covering the whole surface of a CMOS image sensor (), as shown in the left image of Fig. 3(b). The middle and right images of Fig. 3(b) show the top-view photographic image of the whole metalens array and the zoomed-in image of the metalens array, respectively. By simply switching the polarization station of incident light, we can obtain two independent images of the USAF resolution test chart taken by the imaging device, as displayed in Fig. 3(c). Figure 3(d) shows a stitched image by combining two sets of subimages after certain image processing (the detailed proceeding is provided in Sec. S5 of the Supplementary Material), of which the region in the red circle corresponds to the FOV of an Olympus microscope with a objective (). The stitched image has a wide FOV area the same as the metalens array region () with the same resolution of a single metalens (, g8e2). Here, we calculated the space-bandwidth product (SBP) to compare the performance of the chip-scale PMID and traditional Olympus microscope, which refers to the effective number of pixels required to represent an image and is a measure of the information content of the imaging system.44 The SBP of the chip-scale PMID is , which is of the traditional microscope with a objective. Figure 3(e) shows the zoomed-in image with the same imaging resolution taken through the PMID (right) and a commercial Olympus microscope (left), respectively. The performance of this PMID is comparable to that of the traditional microscope with the same resolution, and it evidently shows the great improvement ( times) in FOV of PMID without decreasing the imaging quality.
Figure 3.Imaging of the chip-scale PMID. (a) Phase distribution of part of the chip-scale metalens in the
This chip-scale PMID is further employed to image different biospecimens. To observe the cells in the solution, we made a cell solution container with glass cover (0.13 to 0.16 mm) and ultraviolet (UV) adhesive, of which the diagram and photographic image are displayed in Figs. 4(a) and 4(b), respectively. Figure 4(c) shows the stitched image of human umbilical vein endothelial cells (HUVECs) in a solution at . This stitched image covers times the area of a singlet metalens without decreasing the resolution. Next, we changed the working wavelength from 450 to 510 nm to derive DOF information owing to the spectral zooming effect. Zoomed-in images of the HUVECs at different operating wavelengths are shown in Fig. 4(d) from which different microscopic cell information at different depths in the solution can be distinguished. According to our characterization, the total DOF is measured at about . (More details are provided in Sec. S6 of the Supplementary Material.) Note that compared with the previous version of the metalens microscope,36 this PMID integrating the CPF gives rise to a high SNR and imaging quality and makes it possible to achieve broadband imaging to resolve the DOF information in a wide-field scheme. So far, based on this chip-scale PMID we have achieved good resolution, high SNR, large FOV, and tunable DOF imaging simultaneously. Moreover, this device also enables wide view living videos for microscopic observations; see Video 1 for flowing HUVECs in a solution as an example.
Figure 4.Wide-field and DOF imaging. (a) Schematic of the cell solution container. (b) Top-view photographic image of the cell solution container. Scale bar: 5 mm. (c) Stitched image of HUVECs in solution. (d) Zoomed-in images of the red box in (c) at 450, 470, 490, and 510 nm, respectively. Scale bar:
To further demonstrate the advantage of the chip-scale PMID for integration, we implemented a miniaturized compact prototype with a combination of the LED light source, liquid crystal polarizer, and sample holder as its image shown in Fig. 5(b). It has a very compact size of , with a 1000-fold reduction in size and weight as compared with the bulky Olympus microscope [see Fig. 5(a)], so it is termed as a portable microscope system (PMS). Note that in this PMS, a liquid crystal (LC) is adopted together with a polarizer and blue-light LED () source to provide a non-mechanical switch of illumination polarization. Based on this PMS, a wide-field microscopic image can be achieved as well. For example, Fig. 5(c) shows the results of the pine stem cross section (c.s.), where all feature sizes can be clearly distinguished with a high imaging quality by comparing with the image (decolored) taken by the Olympus microscope (). Meanwhile, the full FOV of the PMS is times the FOV of the Olympus microscope camera with a objective. Undoubtedly, this PMID-enabled PMS prototype has shown its overwhelming advantage in portable and convenient microscope imaging. There are more details about PMS in Sec. S7 of the Supplementary Material and Video 2.
Figure 5.Compact prototype of the chip-scale PMID. (a) Photographic image of the traditional Olympus microscope together with the PMS prototype. (b) Photographic image of the PMS prototype (
6 Discussion and Conclusion
We have proposed a PMID for wide-field and DOF imaging via integrating both metalenses and CPF directly to a CMOS image sensor. A new kind of polarization-multiplexed metalens was proposed to modulate the co- and cross-polarized light independently, which rightly enables the fixed polarization filter to work for the integrated device. Detailed characterizations of imaging performances of the singlet metalenses, including the SNR, MTF, resolution, and imaging bandwidth were systematically performed and carefully compared with the counterpart without CPF. As a result, we successfully demonstrated a chip-scale PMID with the resolution of , an FOV of , and DOF of , verifying the advantage of wide-field and DOF imaging over the traditional microscope at the same resolution. To the best of our knowledge, this is the first metalens microscope that has an FOV larger than the mature traditional ones, and the DOF tuning without mechanical motion is also a striking feature in large FOV imaging. In the meantime, the miniaturized PMS prototype has a 1000-fold reduction in volume and weight of the traditional. It should be mentioned that the current resolution () of PMID is not limited by the metalens but the pixel size () of the CMOS image sensor in our imaging system, which would possibly be improved by employing a smaller pixel-sized CMOS sensor with further development of CMOS technology. The other possibility of improving the resolution could be the booming computational imaging techniques, which have been proved to be an efficient method in many cases of upgrading the imaging performance. Frankly, our microscope imaging function based on two circularly-polarized light will be affected with respect to the circular-polarization sensitive objects, in which some subimages will be possibly weaken or even absent. For these cases, there is still an alternative solution by changing the metalens array design from the circular polarization multiplexing to the linear polarization multiplexing (i.e., - and -polarizations), then it is valid to work in the circular polarization-sensitive objects.21 This chip-scale microscope has shown its overwhelming advantages not only in large FOV and DOF but also the compactness compared with the traditional optical microscope, which will undoubtedly illuminate new revolutionary optical devices by meta-technology.
Xin Ye is a PhD student working under the supervision of Tao Li at the College of Engineering and Applied Sciences at Nanjing University. She received her BS degree from the University of Shanghai for Science and Technology, Shanghai, China, in 2019. Currently, she is working on metasurface and advanced imaging.
Xiao Qian is a master student working under the supervision of Tao Li at the College of Engineering and Applied Sciences at Nanjing University. He received his BS degree from Anhui University in 2020. Currently, he is working on metasurface devices.
Yuxin Chen is a master student working under the supervision of Tao Li at the College of Engineering and Applied Sciences at Nanjing University. He received his BS degree from Nanjing University in 2019. Currently, he is working on nanofabrications.
Rui Yuan is a PhD student working under the supervision of Wei Hu at the College of Engineering and Applied Sciences at Nanjing University. He received his MS degree in physics from Hebei University of Technology, Tianjin, China, in 2018. His current research interests include liquid crystal-based beam steering, optical phase array, and tunable lens.
Xingjian Xiao is a PhD student working under the supervision of Tao Li at the College of Engineering and Applied Sciences at Nanjing University. He received his BS degree from Nanjing University in 2019. Currently, he is working on advanced imaging and computation.
Chen Chen is an assistant researcher at the College of Engineering and Applied Sciences at Nanjing University. She received her PhD from Nanjing University in 2021. Currently, she is working on metasurface devices.
Wei Hu is currently a professor of optical engineering at Nanjing University. He received his PhD in polymer chemistry from Jilin University, Changchun, China, in 2009. His research interests include liquid crystal materials and optical devices, with a focus on photoalignment-enabled liquid crystalline superstructures, optically addressed spatial light modulators, and liquid crystal telecom/terahertz elements.
Chunyu Huang is an engineer at Collaborative Innovation Center of Advanced Microstructures at Nanjing University. She received her PhD from Jilin University in 2010. Currently, she is working on nanofabrications.
Shining Zhu is a professor at Nanjing University. He received his PhD from Nanjing University in 1996 and is the group leader of Dielectric Superlattice Laboratory. His research interest includes condensed matter optics, quasiphase matching physics and nonlinear optics, optoelectronic functional materials, quantum optics, and metamaterials.
Tao Li is a professor at the College of Engineering and Applied Sciences at Nanjing University. He received his PhD from Nanjing University in 2005. His research interest includes metamaterials, plasmonics, and nanophotonic integrations.
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