• Journal of Innovative Optical Health Sciences
  • Vol. 18, Issue 6, 2550024 (2025)
Zhijia Liu1, Duantao Hou1, Yiyan Fei1, Lan Mi1、*, and Jiong Ma1、2、3、**
Author Affiliations
  • 1[in Chinese]
  • 2[in Chinese]
  • 3[in Chinese]
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    DOI: 10.1142/S1793545825500245 Cite this Article
    Zhijia Liu, Duantao Hou, Yiyan Fei, Lan Mi, Jiong Ma. Active-modulated fluorescence fluctuation super-resolution microscopy with multi-resolution analysis[J]. Journal of Innovative Optical Health Sciences, 2025, 18(6): 2550024 Copy Citation Text show less

    Abstract

    A new scheme of super-resolution optical fluctuation imaging (SOFI) is proposed to broaden its application in the high-order cumulant reconstruction by optimizing blinking characteristics, eliminating noise in raw data and applying multi-resolution analysis in cumulant reconstruction. A motor-driven rotating mask optical modulation system is designed to adjust the excitation light field and allows for fast deployment. Active-modulated fluorescence fluctuation super-resolution microscopy with multi-resolution analysis (AMF-MRA-SOFI) demonstrates enhanced resolution ability and reconstruction quality in experiments performed on sample of conventional dyes, achieving a resolution of 100nm in the fourth order compared to conventional SOFI reconstruction. Furthermore, our approach combining expansion super-resolution achieved a resolution at 57 nm.
    Zhijia Liu, Duantao Hou, Yiyan Fei, Lan Mi, Jiong Ma. Active-modulated fluorescence fluctuation super-resolution microscopy with multi-resolution analysis[J]. Journal of Innovative Optical Health Sciences, 2025, 18(6): 2550024
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