Hao Yang, Shengbing Shi, Liangliang Yao, Dian Gui, Lu Shi, Jinyu Zhao, Haoran Meng. Microorganism Contour Segmentation Method Using Imaging Models and Fourier Descriptors[J]. Laser & Optoelectronics Progress, 2025, 62(2): 0237007
- Laser & Optoelectronics Progress
- Vol. 62, Issue 2, 0237007 (2025)
Fig. 1. Underwater in situ holographic imaging system. (a) System optical path structure; (b) schematic of optical path; (c) physical drawing
Fig. 2. Partial holograms captured by the system
Fig. 3. Reconstruction results of hologram. (a) Pre-reconstruction resolution plate image; (b) post-reconstruction resolution plate image; (c) pre-reconstruction biological specimen image; (d) post-reconstruction biological specimen image; (e) pre-reconstruction underwater microbial 1 image; (f) post-reconstruction underwater microbial 1 image; (g) pre-reconstruction underwater microbial 2 image; (h) post-reconstruction underwater microbial 2 image
Fig. 4. Comparison of interference fringe filtering spectra. (a) Spatial domain image before filtering; (b) frequency domain image before filtering; (c) spatial domain image after filtering by traditional method; (d) frequency domain image after filtering by traditional method; (e) spatial domain image after filtering by improved method; (f) frequency domain image after filtering by improved method
Fig. 5. Interference stripe filtering effect. (a) Resolution plate image before filtering; (b) resolution plate image after filtering; (c) biological specimen image before filtering; (d) post-filtered biological specimen image; (e) pre-filtered biological specimen image; (f) post-filtered biological specimen image
Fig. 6. Underwater microbial contour segmentation network architecture
Fig. 7. Structure diagram of background scattering estimation module
Fig. 8. Structure diagram of direct transmission estimation module
Fig. 9. Network structure of contour segmentation task
Fig. 10. Local refinement algorithm
Fig. 11. Image fusion effect. (a) (b) (c) Images without water; (d) (e) (f) images with water; (g) (h) (i) annotation
Fig. 12. Comparison of contour representation capabilities
Fig. 13. Experimental errors. (a) Regularly shaped microorganisms; (b) irregularly shaped microorganisms
Fig. 14. Comparison of spatial continuity
Fig. 15. Errors of spatial continuity
Fig. 16. Segmentation results of different algorithms
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Table 1. Confusion matrix
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Table 2. Comparison of experimental results
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