In order to improve the sensitivity, specificity and safety of latent fingermark reagent based on nano technique, we designed the structure of ZnO/PAMAM G5.0 nano composites, explored its synthesis method, examined the fluorescent property of the newly developed nano composites and investigated its application on the enhancement of cyanoacrylate pre-treated fingermark. The results indicated that ZnO nano particles could be on-site synthesized under the control of PAMAM G5.0 dendrimer template. ZnO/PAMAM G5.0 nano composites emitted blue light under the excitation of long UV light. When the concentration ratios of Zn2+ and PAMAM dendrimers were between 10～100, the fluorescent intensity of ZnO/PAMAM G5.0 nano composites changed with respect to the increase of the concentration ratio. The highest fluorescent intensity could be reached when the concentration ratio was set at 60. Cyanoacrylate pre-treated fingermark could be enhanced by the amidation reaction between ZnO/PAMAM G5.0 nano composites and the ester terminal groups of cyanoacrylate polymer. The newly developed ZnO/PAMAM G5.0 nano composites obtain the fluorescent characteristics through non-toxic quantum dots and strengthen latent the fingermark development based on routine developing method. It will play an important role in the development of latent fingermark reagent based on nano technique in the near future.