To explore the effects of long non-coding ribonucleic acid (LncRNA) MAGI2-AS3 in regulating the miR-143-3p/PTP4A1 axis on cervical cancer (CC) cells, the expressions of MAGI2-AS3, miR-143-3p and PTP4A1 in the resected CC tissues and adjacent tissues of 54 patients with CC during the operation were detected by RT-qPCR. HeLa cells were randomly divided into control group, sh-NC group, sh-MAGI2-AS3 group, sh-MAGI2-AS3+anti-NC group and sh-MAGI2-AS3+anti-miR-143-3p group. The expression of MAGI2-AS3, miR-143-3p and PTP4A1 in each group was measured. Cell proliferation, migration, and invasion, expressions of PTP4A1, matrix metalloproteinase-2 (MMP-2), MMP-9 and proliferating cell nuclear antigen (PCNA) were detected. The effects of MAGI2-AS3 knockout on CC tumor growth and PTP4A1 expression were verified by animal experiments. The results showed: the expression levels of MAGI2-AS3 and PTP4A1 mRNA in CC tissues was 1.97±0.35, 1.73±0.34, higher than (1.02±0.33, 0.98±0.31) in adjacent tissues, and the expression levels of miR-143-3p was 0.48±0.14, lower than (1.01±0.16) in adjacent tissues (P<0.05). Compared with the sh-NC group and the control group, the A_{490 nm} value, EdU positive cell rate, scratch healing rate, invasion number, mRNA expression levels of MAGI2-AS3 and PTP4A1, and protein expression levels of PCNA, PTP4A1, MMP-2 and MMP-9 in HeLa cells of the sh-MAGI2-AS3 group were all decreased; the mRNA expression level of miR-143-3p increased (P<0.05). Compared with the sh-MAGI2-AS3 group and the sh-MAGI2-AS3+anti-NC group, the mRNA expression level of miR-143-3p in the sh-MAGI2-AS3+anti-miR-143-3p group decreased, the A_{490 nm} value, the rate of EdU positive cells, the scratch healing rate, the number of invasions, as well as the mRNA expression levels of PTP4A1 and the protein expression levels of PCNA, PTP4A1, MMP-2 and MMP-9 increased (P<0.05). Compared with the sh-NC group, the mass, volume, positive rate of Ki-67 and positive rate of PTP4A1 of the transplanted tumors in the sh-MAGI2-AS3 group were all decreased (P<0.05). To sum up, MAGI2-AS3 targeted negative regulation of miR-143-3p; miR-143-3p negatively regulates PTP4A1. Knockout of MAGI2-AS3 may inhibit the proliferation, migration and invasion of CC cells, which may be achieved by regulating the miR-143-3p/PTP4A1 axis.