Contents
2019
Volume: 28 Issue 3
12 Article(s)

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Lens-free Microscopy
XU Xinzhu, GUAN Meiling, JIANG Shan, YANG Houpu, WANG Shu, and XI Peng
Lens-free microscopic technology utilizes Gabor-based in-line holographic optical path to capture the interference images generated by illuminating lights through the micro-samples, with the help of an area array detector but without any imaging lenses. It is a digital microscopic imaging technology which uses a digita
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 193 (2019)
Heterologous Expression and Activity of Rhamnolipid Gene Regulated by Three Different Constitutive Promoters in Pseudomonas protegens
XIE Zhiling, CHEN Hanna, ZHONG Lin, XU Jiaying, LI Yan, PAN Deng, LIU Feng, DING Xuezhi, XIA Liqiu, ZHANG Youming, and TU Qiang
In this research, the recombinant expression vector pBBR1-genta-RhlAB containing the rhamnosyltransferase gene (RhlAB)was modified via Red/ET homologous recombineering. Then, three recombinant expression vectors pBBR1-genta-Papra-RhlAB, pBBR1-kan-Ptac-RhlAB and pBBR1-kan-PrhaB-RhlAB with three different constitutive pr
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 229 (2019)
Real-Time Monitoring of Viscoelastic Changes of Neonatal Rat Primary Cardiomyocytes Subjected to the Treatments of Different Concentrations of Inotropic Drugs Using Quartz Crystal Microbalance
PENG Han’ge, ZHOU Tiean, TAN Chengfang, and TU Boxuan
The quartz crystal microbalance (QCM)was used to monitor the dynamic adhesions of neonatal rat primary cardiomyocytes and the subsequent viscoelastic changes induced by different concentrations of inotropic drugs onto the gold crystal electrodes in a non-invasive and real-time manner. The adhesion of neonatal rat prima
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 239 (2019)
Establishment and Characteristics Analysis of in vitro Culture An Ear Fibroblast Cell Line Derived from Hartley Guinea Pig
CHEN Ruiping, WU Chuman, ZHOU Wenyi, JIANG Haodong, WU Yue, PENG Junwei, GUO Huiming, HUANG Huanlei, ZHU Shuoji, ZHAO Mingyi, and ZHU Ping
This experiment was performed to rapidly obtain fibroblasts from guinea pig’s ears, and provide initiating cells for somatic cell reprogramming. The ear tissues were minced and digested with 0.05% Trypsin-EDTA and Collagenase IV. The cells were cultured in vitro and morphologically observed. The cells were detected for
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 245 (2019)
The ISSR Analysis of 24 Dendrobium officinale Germplasm Resources
LU Yan, PAN Lite, LI Huili, LIN Xiaoxia, ZHOU Ping, GUO Xinhong, and YIN Liping
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 252 (2019)
Construction of Zebrafish gpr98 Gene Knockout Experimental Model
DENG Ming, ZENG Yuqian, LIU Le, GUO Jifeng, DENG Yun, and TANG Beisha
gpr98 is associated with a variety of diseases, and it is highly conserved in human and zebrafish. Constructing the gpr98 gene mutant strain of zebrafish can provide an excellent animal model and research basis for explaining the function of the gpr98 gene. In this paper, two target sites with a distance of 42 bp in ex
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 258 (2019)
Mutation Rates at 18 X-chromosome STRs in the North China Han Population
YU Zhengliang, ZHANG Xiaofang, LIU Hong, SHANG Lei, ZHANG Jian, ZHAO Xingchun, LIU Chao, and LI Wanshui
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 270 (2019)
Screening and Agronomic Analysis of Mutants from Indica Maintainer Rice T98B Treated with γ-Ray Irradiation
TAN Yanning, YANG Zhen, and YUAN Dingyang
Radiation mutagenesis is available for crops germplasm creation. In this study, gamma-ray irradiation was used to induce the rice cultivar T98B which is a main maintainer line of hybrid rice, under the treatment of mature seeds at a dose of 300 Gy. It focused on screening and grouping of agronomic mutants, and the freq
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 274 (2019)
A Study on the Effects of Cultivation Factors on Cotton Yield in Short Season
YANG Huibing, YANG Junxing, ZHOU Zhonghua, and XU Na
The long growing period of cotton in the Yangtze river basin is not conducive to the planting of cotton oil in the double cropping system. By regulating cultivation factors, the cotton growing period can be shortened, so as to realize cotton oil single cropping and improve the planting efficiency.Early maturing cotton
Acta Laser Biology Sinica
  • Publication Date: Aug. 07, 2019
  • Vol. 28, Issue 3, 281 (2019)
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