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2020
Volume: 29 Issue 2
12 Article(s)
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Applications of Terahertz Technology on Biomedicine
CHEN Xiaowan, and JIANG Linhua
In order to meet the requirements of sustainable growth and development for human beings, biomedical research has always been our focus. Terahertz(THz)has been widely used in biomedicine because of its own advantages. This article presents the applications of THz characterization techniques and THz biological effects iIn order to meet the requirements of sustainable growth and development for human beings, biomedical research has always been our focus. Terahertz(THz)has been widely used in biomedicine because of its own advantages. This article presents the applications of THz characterization techniques and THz biological effects in biomedicine. The applications of THz characterization technology are divided into five parts: amino acid and polypeptide, DNA, protein, the detection of cancer and other applications such as caries detection. The researches of THz biological effects mainly concentrate on the effects of THz on the tissues and cells. Furthermore, this article briefly introduces chemometrics method and its applications in biomedical applications in combination with THz technology. In this review, we summarize the drawbacks of THz technology in biomedical field and discuss the direction of further research. This article reviews the applications of THz technology in biomedicine, and establishes the foundation for relevant study..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 97 (2020)
Application of Fluorescence Competitive Binding Technology in Detection of the Binding Ability of Insects Olfactory Related Proteins
PENG Zhu, HUANG Li, and ZHAO Huiting
Olfactory related proteins of insects play an important role in their behaviors and physiological activities such as foraging, mating, defense, spawning, information exchange and habitat selection. Fluorescence competitive binding assay is a typical method for detecting the binding properties of insect OBPs and CSPs wiOlfactory related proteins of insects play an important role in their behaviors and physiological activities such as foraging, mating, defense, spawning, information exchange and habitat selection. Fluorescence competitive binding assay is a typical method for detecting the binding properties of insect OBPs and CSPs with environmental odor molecules. In this paper, some researches and applications on fluorescence competitive binding assay used for insects olfactory proteins (OBPs and CSPs) are reviewed. It provides theoretical reference for further exploration on insects olfactory recognition mechanism and formulation strategies on pests control and economic insects protection..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 106 (2020)
A Study on the Application of Macleaya cordata in Livestock Production
ZHANG Lingmei, ZHU Jinjia, YANG Lingyuan, and TANG Shaoxun
Macleaya cordata is a kind of Chinese herbal medicine plant, the alkaloids extracted from Macleaya cordata have strong anti-inflammatory, bactericidal and anti-tumor functions. Alkaloids plays an important role in enhancing animal immunity, improving animal growth performance and feed conversion rate in livestock and pMacleaya cordata is a kind of Chinese herbal medicine plant, the alkaloids extracted from Macleaya cordata have strong anti-inflammatory, bactericidal and anti-tumor functions. Alkaloids plays an important role in enhancing animal immunity, improving animal growth performance and feed conversion rate in livestock and poultry production. This article summarized the main components, separation and extraction methods, toxicity and pharmacological activities, and the application status in livestock and poultry production, in order to provide theoretical basis for the rational application of Macleaya in livestock and poultry production..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 113 (2020)
Interspecies Utilization of Mouse ACE2 as a Receptor for Bat SARS-like Coronavirus WIV1
WANG Qiong, LI Dan, LI Jinyan, HU Bingjie, QIU Ye, and GE Xingyi
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a highly pathogenic virus which originated from bat SARS-like coronavirus (SL-CoV) and infects humans. Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, is a key molecular affecting SARS-CoV pathogenesis, host range, and cross-specieSevere acute respiratory syndrome coronavirus (SARS-CoV) is a highly pathogenic virus which originated from bat SARS-like coronavirus (SL-CoV) and infects humans. Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, is a key molecular affecting SARS-CoV pathogenesis, host range, and cross-species transmission. Previous studies demonstrated that SARS-CoV and some SL-CoV strains (such as WIV1) can use human, civet, and bat ACE2 to enter cells, while SARS-CoV utilizes mouse ACE2 less effectively. Though rodents are the most diverse mammals and the most popular experimental model, the utilization of mouse ACE2 by SL-CoV has not been revealed. In the present study, pseudoviral infection experiments were conducted to compare SARS-CoV BJ01 and SL-CoV WIV1 on their ability of using human, civet, bat, mouse ACE2 and ACE2 mutants to enter cells. Meanwhile, the binding between receptor-binding domain (RBD) of the two viruses and different ACE2 was verified by protein binding experiments. The results showed that SL-CoV WIV1 can use mouse ACE2 to enter cells, and WIV1 RBD binds to mouse ACE2 as efficiently as human and civet ACE2 whereas more strongly than bat ACE2. The L440P mutation of ACE2 in different species can significantly reduce the ability of RBD binding and the efficiency of pseudovirus invasion. The studies indicate that mouse ACE2 is a functional receptor for SL-CoV WIV1 and L440 of ACE2 is a key amino acid site affecting interspecies infection. The study is helpful to further understand the receptor recognition and cross-species transmission mechanism of SL-CoV, and will be beneficial to the prevention and control of similar CoV in the future..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 120 (2020)
An Experiment Study on Inactivation of HL60 Cells by BiFeO3@TiO2 Composite Nanoparticle Photodynamic Therapy in vitro under Weak Stable Magnetic Field
ZHAO Yang, LIU Liling, ZHANG Qiyun, FANG Jie, CHEN Li, AI Baoquan, and XIONG Jianwen
In this paper, BiFeO3@TiO2 composites nanoparticles were synthesized by sol-gol method. The nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD), fluorescence emission spectrum etc. The Cell Counting Kit-8 (CCK-8) method was used to detect the activity of HL60 cells treateIn this paper, BiFeO3@TiO2 composites nanoparticles were synthesized by sol-gol method. The nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD), fluorescence emission spectrum etc. The Cell Counting Kit-8 (CCK-8) method was used to detect the activity of HL60 cells treated with a final concentration of 50 μg/mL nanoparticles under the action of weak stable magnetic field with different strengths in dark room and light conditions. The experimental results showed that the viability of HL60 cells treated with BiFeO3@TiO2 composites nanoparticles for 12 hours at a concentration of 50 μg/mL increased from 78% to 85%, with the increment of TiO2 shell thickness in the darkroom condition. However, cell groups treated by BiFeO3@TiO2 composites nanoparticles with a mass ratio of BiFeO3 to TiO2 of 1∶2 showed the highest cell inactivated efficiency of 78% under light exposure. The weak stable magnetic field enhanced the PDT inactivation efficiency of HL60 cells.Therefore,it provides a reference for the clinical application of photodynamic therapy in the treatment of tumor cells under weak stable magenetic field..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 128 (2020)
Photodynamic Therapy Based on Feature Extraction of Lesion Images
MEI Qisheng, CHEN Yufang, WU Zhencong, and WANG Ming
The feature recognition and extraction of lesions has always been a classic problem in the field of medical image processing. The feature extraction of lesion images is to transform the original image into a set of characteristic images with obvious physical meaning and statistical significance. Identifying the lesion The feature recognition and extraction of lesions has always been a classic problem in the field of medical image processing. The feature extraction of lesion images is to transform the original image into a set of characteristic images with obvious physical meaning and statistical significance. Identifying the lesion area and analyzing the condition of the lesion area is an important part of photodynamic therapy. Taking the red spot disease as an example, photodynamic therapy uses light, photosensitizer and molecular oxygen to exert a strong photodynamic effect on the diseased cells, allowing the active oxygen to kill the diseased cells, and finally achieve the purpose of treating the disease. Traditional treatment options have problems such as inability to accurately diagnose the lesion area, inability to accurately treat, and high overall treatment costs. This study designed a narrow-spectrum LED light source for irradiation treatment of port wine stains, and calculated the distribution of lesions and the degree of lesions according to the characteristics of the lesions. Finally, based on the calculation results, the film with non-uniform transmission density was used to precisely control the intensity of the treatment light source. The experimental results show that the image feature extraction technology, the designed light source and the film can solve the problems of inaccurate diagnosis, inaccurate treatment and treatment source uniformity, and have broad application prospects in the treatment of port wine stains described in the experiment..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 142 (2020)
Analysis of DAPI Staining in Different Tissues of Arabidopsis
YU Jingfang, YIN Runzhu, and ZHOU Jun
4’,6-diamidino-2-phenylindole (DAPI), as one of specific DNA fluorescent dyes, is widely used in fluorescence microscopic observation of various biological cells. However, there is still lack of a systematic characterization of different tissues response to DAPI staining in plant. In this study, using the model plant A4’,6-diamidino-2-phenylindole (DAPI), as one of specific DNA fluorescent dyes, is widely used in fluorescence microscopic observation of various biological cells. However, there is still lack of a systematic characterization of different tissues response to DAPI staining in plant. In this study, using the model plant Arabidopsis thaliana, we explored the differential responses to DAPI staining in various tissues. Firstly, we generated a stable nucleus-targeted transgenic plant UBQ10∶BAG5-eYFP. The results showed that only the epidermal cells and mesophyll cells can be stained by DAPI in living plant. Compared with chloroplast, the DNA in mitochondria is very sensitive to DAPI staining, displaying a good co-localization between the fluorescence of DAPI and mitochondrial marker CoxIV-RFP. After fixed with paraformaldehyde, the root meristem zone and stomatal guard cells can be stained by DAPI. These results indicated that different tissues have great differences when being stained with DAPI in Arabidopsis. The leaf epidermal cells and mesophyll cells would be good models for studying the function of nucleus in living plant..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 148 (2020)
Bone Marrow-derived Mesenchymal Stem Cell Culture Medium Promotes STAT3 Phosphorylation and Induces Polarization of Raw264.7 Cells to M2 Type
LI Zhiwei, ZHOU Haoyue, LIU Xiangyue, HE Yi, DING Xiaofeng, and HU Lingyu
The occurrence of inflammatory diseases is the focus of today’s clinical medicine. M1 type macrophages induce the inflammatory response and release proinflammatory mediators while M2 type inhibit inflammation via secreting anti-inflammatory factors. The polarization of M1 type macrophages to M2 type changes from an infThe occurrence of inflammatory diseases is the focus of today’s clinical medicine. M1 type macrophages induce the inflammatory response and release proinflammatory mediators while M2 type inhibit inflammation via secreting anti-inflammatory factors. The polarization of M1 type macrophages to M2 type changes from an inflammatory state to a state in which inflammation is inhibited. Therefore, studying the M2 type polarization of macrophages to relieve inflammation will be beneficial to the treatment of inflammatory diseases. To explore the molecular mechanism of bone marrow-derived mesenchymal stem cell culture medium (BMSC-CM) to induce the Raw264.7 cell differentiation towards M2 macrophages, bone marrow-derived mesenchymal stem cells were derived from 3 week-old C57BL/6 mice. We induced Raw264.7 cells to M1 macrophage with lipopolysaccharide (LPS, 1 μg/mL). Then we cultured these Raw264.7 cells in culture mediums which were previously used to culture bone marrow-derived mesenchymal stem cells. The mRNA relative expression of TNF-α, INOS, ARG-1, TGF-β1 and IL-10 was detected by semi-quantitative PCR. Western blotting was used to detect the expression level of proteins involved in the STAT3 signaling pathway. After cultured in BMSC-CM and induced by LPS, M2 markers (ARG-1, TGF-β1) and IL-10 of the Raw264.7 cells was increased and the p-STAT3 protein level was promoted significantly. The results show that BMSC-CM can induce Raw264.7 cells differentiation towards M2 macrophages by promoting STAT3 phosphorylation via IL-10/STAT3 signaling pathway..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 153 (2020)
Bioinformatics Analysis of the Gene Expression Profile in Chronic Rhinosinusitis with Nasal Polyps
CHEN Gang, YU Yang, and WANG Line’e
The data-set of chronic rhinosinusitis expression profiles was downloaded from NCBI sub-database Gene Expression Omnibus. The differentially expressed genes were analyzed by GEO2R. The DEGs were analyzed and shown by the volcano plot. The GO analysis and KEGG analysis were performed by using David online tool.The DEGs The data-set of chronic rhinosinusitis expression profiles was downloaded from NCBI sub-database Gene Expression Omnibus. The differentially expressed genes were analyzed by GEO2R. The DEGs were analyzed and shown by the volcano plot. The GO analysis and KEGG analysis were performed by using David online tool.The DEGs were introduced into the String online data-base for analysis and the differential gene interaction network map was drawn.The interaction network data was imported into Cytoscape software, which can select Hub genes. The key sub-network was analyzed. A total of 699 DEGs were screened, of which 475 genes were up-regulated genes and 224 genes were down-regulated genes.The GO analysis showed that up-regulated DEGs were enriched in biological processes significantly,including inflammatory response, immune response,positive regulation of inflammatory response,chemotaxis and cell adhesion. The down-regulated DEGs were enriched in saliva secretion, biomineral tissue development, cellular amino acid biosynthetic process, retina homeostasis and ion transmembrane transport.The KEGG pathway analysis showed that up-regulated DEGs were enriched in hematopoietic cell lineage, cytokine-cytokine receptor interaction,osteoclast differentiation,chemokine signaling pathway. The down-regulated DEGs were enriched in salivary secretion, bile secretion.The top 10 Hub genes contained ITGAM, IL10, CD86, TLR8, ITGAX, CCL2, CCR7, SRC, EGF and ITGB2, which were identified from PPI network. The results provide a comprehensive bioinformatics analysis of DEGs in chronic rhinosinusitis with nasal polyps, however, more fundamental research is needed to validate the findings.Not only can the conclusion provide a new idea for the research direction of chronic rhinosinusitis and nasal polyps,but also make a suggestive effect on the pathogenesis of nasal polyps..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 161 (2020)
Analysis of Bacterial Community Diversity of Spoiled Egg Contents
SHEN Tianyu, CHEN Lili, LIU Yan, LIU Jin, CHEN Huan, ZHANG Lizhao, and JIANG Liwen
To investigate the diversity and structure of bacterial community in the contents of spoiled eggs. Samples were collected from egg protein liquates (J) and smelly eggs (JC), and the 16S rDNA (V4 V5) region amplicons were sequenced by Miseq. The results showed that 42 550 and 38 477 high-quality sequences were obtained To investigate the diversity and structure of bacterial community in the contents of spoiled eggs. Samples were collected from egg protein liquates (J) and smelly eggs (JC), and the 16S rDNA (V4 V5) region amplicons were sequenced by Miseq. The results showed that 42 550 and 38 477 high-quality sequences were obtained from J and JC, respectively, which were assigned to 19 OTUs, covering 2 phyla, 14 families, 19 genus and 23 species. The J samples had slightly lower bacterial richness, whereas higher community diversity than JC. The dominant taxa were Proteobacteria Enterobacteriaceae Citrobacter, Serratia and Moraxellaceae Acinetobacter, Pseudomonadaceae Pseudomonas, Firmicutes Bacillaceae Bacillus and Carnobacteriaceae Carnobacterium; The relative abundance of Bacillus was the highest (40.10%) in J samples, whereas unclassified at the species level. In JC samples, the relative abundance of Citrobacter was the highest (81.23%), mainly Citrobacter gillenii, and the abundance of Escherichia-Shigella and Enterococcus was very low. There are 14 unclassified or uncultured, and the same species belonged to different OTU in 23 species at the species level. The bacterial population of spoiled eggs is complex, and its diversity of community and abundance have an effect on the spoilage of eggs, which can provide a reference for the further study of the main spoilage bacteria of eggs and their control..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 168 (2020)
The Establishment of the Zebrafish miR-196a-1 Knockout Lines
ZENG Ting, FU Guifang, XIE Binling, DU Han, XIE Huaping, and YIN Yulong
MicroRNA (miRNA), a type of endogenous non-coding RNA with a length of about 19~23 nucleotides, participates in post-transcriptional regulation of gene expression by affecting the stability and translation of mRNA. Bioinformatics analysis showed that the gene is highly conserved in various species. In order to study thMicroRNA (miRNA), a type of endogenous non-coding RNA with a length of about 19~23 nucleotides, participates in post-transcriptional regulation of gene expression by affecting the stability and translation of mRNA. Bioinformatics analysis showed that the gene is highly conserved in various species. In order to study the role of miR-196a-1 gene in intestinal development, we used cloning free CRISPR/Cas9 gene editing technology to establish the zebrafish miR-196a-1 knockout lines. Firstly, two knockout sites of zebrafish miR-196a-1 gene were screened by online analysis, the two knockout sites were 132 bp apart. Next, the template guide DNA of miR-196a-1 gene was amplified by PCR. Then, the template DNA was transcribed into the sgRNA of miR-196a-1 gene. Finally, the sgRNA of miR-196a-1 gene and Cas9 protein were co-injected into zebrafish embryos at the 1-cell stage. The effectiveness of gene editing was tested after 36 hpf.The results showed that there was a deletion of 102 bp base in miR-196a-1 gene, revealing that the CRISPR/Cas9 system is effective in knocking out miR-196a-1 gene. The miR-196a-1 knockout line of zebrafish was established successfully after screening the F0, F1, and F2 generations. These findings laid a foundation for exploring the role of miR-196a-1 in zebrafish intestinal development..
Acta Laser Biology Sinica
- Publication Date: Jan. 27, 2021
- Vol. 29, Issue 2, 176 (2020)
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