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Research Progress of Wide-field Optical Coherence Tomography in Ophthalmology
TAO Chenglong, YANG Biwen, FAN Ranran, ZHONG Huiqing, SU Chengkang, GUO Zhouyi, and LIU Zhiming
Wide-field optical coherence tomography (WF-OCT) is a new direction in the field of optical coherence tomography. It receives interference signals to obtain two-dimensional information of the sample, and establishes the image of the whole section of the sample at one time, so as to obtain the image information of the sWide-field optical coherence tomography (WF-OCT) is a new direction in the field of optical coherence tomography. It receives interference signals to obtain two-dimensional information of the sample, and establishes the image of the whole section of the sample at one time, so as to obtain the image information of the sample quickly and efficiently, and to realize the research on the characteristics of the object. In this paper, the basic principle, basic structure and characteristics of WF-OCT system, and its applications in ophthalmology in recent years are summarized, which can provide ideas and reference for the instrument design of WF-OCT and its ophthalmic applications..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 97 (2021)
Research Progress of Optogenetics in the Treatment of Neurological Diseases
WANG Yunxi, XUE Qingping, FENG Xixi, XING Guogang, and XU Fan
Neurology disease is a common clinical disease, its pathogenesis is complex, if without timely intervention, it is readily to lead to the deterioration of the disease, and accompanied by a variety of complications, which seriously endanger the physical and mental health of patients. Therefore, the main task in the fielNeurology disease is a common clinical disease, its pathogenesis is complex, if without timely intervention, it is readily to lead to the deterioration of the disease, and accompanied by a variety of complications, which seriously endanger the physical and mental health of patients. Therefore, the main task in the field of neuroscience is to thoroughly understand the regulation mechanism of brain central nerve cells. In recent years, with the establishment and rapid development of optogenetics, this technology that can enter neurons and precisely control cells provides help for researchers to further explore the pathogenesis of neurological diseases and the treatment of such diseases at the cellular level. From the development history of optogenetics, the discovery of light sensitive protein and the research progress of optogenetics in some common nervous system diseases, this paper expounds the optogenetics, which provides a new method for researchers to explore the clinical treatment of neurological diseases, and further promotes the human understanding of the role of neurons in the brain..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 105 (2021)
Research on Growth Promoting Factors of Lactic Acid Bacteria
OUYANG Jia, WANG Yu, WU Guang, WANG Jiaxuan, and SU Xiaojun
Lactic acid bacteria are beneficial bacteria in the intestinal tract, which is beneficial to the regulation of human health. Its growth ability and activity are affected by external conditions. Growth promoting-factor is a kind of substance which can promote the growth and reproduction of microorganism and can be used Lactic acid bacteria are beneficial bacteria in the intestinal tract, which is beneficial to the regulation of human health. Its growth ability and activity are affected by external conditions. Growth promoting-factor is a kind of substance which can promote the growth and reproduction of microorganism and can be used to effectively increase the quantity and activity of lactic acid bacteria. In this paper, we review the relationship between lactic acid bacteria growth promoting-factors and prebiotics, as well as their sources, classes and mechanisms of action, and look forward to their future development, in order to provide a reference for the application of growth-promoting factors in the lactic acid bacteria industry..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 110 (2021)
A Review of the Pathogenic Biology of Mycoplasma pneumoniae
LIU Yao, and LIAO Guoyang
Mycoplasma pneumoniae (MP) is a kind bacterial of human pathogen, which could cause primary atypical pneumonia in the worldwide, as well know. According its bacteria size and genome size, MP could be defined as a smaller pathogenic bacteria, which caused both upper and lower human respiratory infections. The patients iMycoplasma pneumoniae (MP) is a kind bacterial of human pathogen, which could cause primary atypical pneumonia in the worldwide, as well know. According its bacteria size and genome size, MP could be defined as a smaller pathogenic bacteria, which caused both upper and lower human respiratory infections. The patients infected MP are also accompanied by various extra-pulmonary infections and other different severe comorbidites. If they are infected by drug-resistant strains or multi-drug resistant strains, they are suffered from higher risk. This review systematically reviewed and analyzed the pathogenic mechanism of MP, the progress on its diagnosis, treatment and vaccine studies, which could provide some basic knowledge of its control, prevention and treatment..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 117 (2021)
Experimental Study on the Inactivation of HL60 Cells by PDT Mediated by Pegylated Black TiO2
ZHANG Qiyun, LIN Shuxin, FANG Jie, LI Miaomiao, XIAO Mucang, AI Baoquan, and XIONG Jianwen
In order to explore the potential of pegylated black TiO2 mediated photodynamic therapy to inactivate HL60 cells, black pegylated TiO2?(PEG@B-TiO2) nanocomposites with different mass ratios were synthesized by the combination of hydrothermal reduction and surface modification method. The physicochemical properties of tIn order to explore the potential of pegylated black TiO2 mediated photodynamic therapy to inactivate HL60 cells, black pegylated TiO2?(PEG@B-TiO2) nanocomposites with different mass ratios were synthesized by the combination of hydrothermal reduction and surface modification method. The physicochemical properties of this prepared samples were characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), ultraviolet and visible spectrum (UV-VIS), fluorescence spectra (FS), transmission electron microscopy (TEM), and granulometer, respectively. Then reactive oxygen species detection kit was used to detect the yield of reactive oxygen (ROS). In order to determine the drug which is most effective, cell proliferation experiment (CCK-8 method) was conducted to compare the toxity of different drugs on HL60 cells in darkroom condition and under visible light irradiation. The results indicated the prepared nanomaterials were with good dispersion, high crystallinity, low electron hole pair recombination rate and strong absorbability to visible light. At the same time, dark toxicity experiment suggested that the biocompatibility was good. Compared with pure TiO2, PEG@B-TiO2 showed higher inactivation efficiency under light irradiation, which manifested that they were expected to be an effective photosensitizer for treating leukemia by photodynamic therapy..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 123 (2021)
Non-invasive Sensing of Denatured Layer on Human Tooth Surface after Er:YAG Laser-ablation Based on Optical Coherence Tomography
ZENG Sujuan, LAN Yintao, GAO Weijian, HUANG Wenyan, SHU Yan, GE Lihong, and ZHANG Jian
The feasibility of quantitatively evaluating the surface character of dentin after Er:YAG laser irradiation by using optical coherence tomography (OCT) is examined. Dentin specimens were prepared from human extracted third molars free of decay. Er:YAG laser non-contact irradiation was performed at pulse duration of 50?The feasibility of quantitatively evaluating the surface character of dentin after Er:YAG laser irradiation by using optical coherence tomography (OCT) is examined. Dentin specimens were prepared from human extracted third molars free of decay. Er:YAG laser non-contact irradiation was performed at pulse duration of 50?μs under water spray. The control groups were sandpaper polishing group and traditional bur treated group. OCT techniques were used to observe the surface properties and measure the changes of surface light intensity of dentin specimens. Histopathological sections were used to analyze the surface structure. Then 37% phosphoric acid was etched for 30 seconds after surface treatment, and the surface morphology was then observed by scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy was used to measure the composition of surface elements. OCT results demonstrated that a layer with high image intensity generated on all experimental dentin surface. Quantitative results obtained that the thickness of these affected layers produced by Er:YAG laser was markedly higher than those of bur- and sandpaper-treated groups (P<0.05). Histology demonstrated that thin denatured layers were created in laser-treated dentin. Little denatured layer on bur- and sandpaper-treated area. The modified layer of histological examination was consistent with the light intensity changes of OCT images. After acid treatment, the dentinal tubules’ entrance enlarged, whereas collagen fibrils network was not exposed in the SEM images of Er:YAG laser groups. After acid treatment for the same time period, EDX spectroscopy showed that the content of Ca and P ion in laser-treated group were higher than bur- and sandpaper-treated groups (P<0.05), which means the laser-treated dentin becomes being more acid resistant. This study demonstrated OCT may become a noninvasive tool to precisely evaluate the character of dentin that would be useful to dentists in helping determine adhesive restoration strategies..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 131 (2021)
Effect of Fruit Peel Water Content and Hardness on the Fruit Laser Marking Quality
SUN Guofeng, CAO Yu, XUE Wei, ZHU Dehua, and LIU Wenwen
The thin-skinned Korla pear and the hard-skinned mangosteen were used as materials. To explore the effects of fruit properties and infrared pulsed laser parameters on the marking effect and fruit storage quality, the influences of fruit peel water content, fruit peel hardness and laser marking speed on the color, line The thin-skinned Korla pear and the hard-skinned mangosteen were used as materials. To explore the effects of fruit properties and infrared pulsed laser parameters on the marking effect and fruit storage quality, the influences of fruit peel water content, fruit peel hardness and laser marking speed on the color, line width and continuity of laser labels were analyzed. And the local morphology, labeling stability and decay of fruit peel marked with optimal processing parameters during storage period were investigated. The results showed that both the water content and hardness of the fruit peel and the laser marking speed had significant influences on the marking effect of thin-skinned and hard-skinned fruits. Besides, the laser labels manifested superior stability during storage with storage quality basically unaffected. It provides a certain reference for exploring the influence of the individual differences of the same kind of fruit and the properties of different kinds of fruit on the laser marking effect..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 138 (2021)
Effects of Compound Chinese Herbal Medicine on AKP and Serum Proteins in Macrobrachium rosenbergii
HONG Dishan, and WANG Yude
To explore the effect of Chinese herbal medicine additive on the immune function of the Macrobrachium rosenbergii, this experiment took Macrobrachium rosenbergii as an object, adding 0%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% compound Chinese herbal additive into the feed, fed for 25 d, under the water temperature 20~30℃ and pH To explore the effect of Chinese herbal medicine additive on the immune function of the Macrobrachium rosenbergii, this experiment took Macrobrachium rosenbergii as an object, adding 0%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% compound Chinese herbal additive into the feed, fed for 25 d, under the water temperature 20~30℃ and pH 7~8. The results showed that as the increase of the concentration of compound Chinese herbal medicine additive in the feed, the growth index, the activity of AKP in serum and the content of the serum protein of Macrobrachium rosenbergii showed a tendency to go up first and then down. The experiment group had greatly improvement compared with the control group. The concentration of Chinese herbal medicine increased the protein content in serum far more than the protein content in muscle did. In each experimental group, the additive amount of 2.5% had the best effect. The experiment showed that adding compound Chinese herbal medicine additive has obvious effects on immune activity and growth performance of Macrobrachium rosenbergii, which can provide basic information for healthy aquaculture of Macrobrachium rosenbergii..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 147 (2021)
Identification, Expression Analysis and Salt Stress Response of Soybean GmWIN1-6 Transcription Factor
CAI Guiping, LIU Baoling, ZHOU Yali, DENG Mimi, GAO Huiling, LI Teng, ZHANG Li, and LI Runzhi
In order to identify an excellent gene source that can be used for soybean genetic improvement, a soybean GmWIN1-6 transcription factor was obtained from soybean (Glycine max) genome identification. Using bioinformatics tools and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technologIn order to identify an excellent gene source that can be used for soybean genetic improvement, a soybean GmWIN1-6 transcription factor was obtained from soybean (Glycine max) genome identification. Using bioinformatics tools and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technology, the physical and chemical properties, protein structure, temporal and spatial expression profile and salt stress of GmWIN1-6 transcription factor were systematically analyzed. The results showed that the open reading frame (ORF) of GmWIN1-6 gene was cloned from soybean flower tissue, its encoded protein consists of 176 amino acids, has a conserved AP2 domain at the N-terminus, and is a hydrophilic protein. In the secondary structure of GmWIN1-6 protein, random coils account for the largest proportion. The tertiary structure is similar to AtWIN1 of Arabidopsis (Arabidopsis thaniana). Phylogenetic analysis showed that GmWIN1-6 is closely related to AtWIN1. The expression of GmWIN1-6 transcription factors in soybean tissues is significantly different. Among them, the expression level is the highest in flower tissues, followed by higher expression levels in the middle and late stages of seed development, and basically coincides with the accumulation period of seed oil. Under salt stress in soybean seedlings, GmWIN1-6 gene up-regulated expression. These experimental findings indicate that the GmWIN1-6 transcription factor may be involved in the regulation of soybean seed development and oil accumulation and seedling stress response, and provide a theoretical basis for soybean genetic improvement and molecular breeding..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 154 (2021)
Genome-wide Association Study of Rice Blast Resistance Genes at the Seedling Stage
CHEN Rubin, PENG Shasha, WANG Dan, and KANG Houxiang
Because the rice varieties with resistance genes are easy to be overcome by pathogens, it is necessary to further explore new rice blast resistance genes. We used 470 germplasm accessions of the Rice Diversity Group II (RDP-II) to evaluate their disease resistance at the rice seedling stage in a hotspot blast nursery (Because the rice varieties with resistance genes are easy to be overcome by pathogens, it is necessary to further explore new rice blast resistance genes. We used 470 germplasm accessions of the Rice Diversity Group II (RDP-II) to evaluate their disease resistance at the rice seedling stage in a hotspot blast nursery (Taojiang, Hunan, China) and identified rice blast resistance loci using genome wide association study (GWAS). Twenty-five varieties with high levels of resistance at the seedling stage were identified which can be used as resistant breeding materials. The disease phenotypes and 700 000 SNP genotypes were used to perform GWAS with the MLM method. A total of 24 resistance-associated loci were identified in the rice genome, which were distributed on 10 chromosomes except chromosome 4 and 7. Among these loci, 5 loci were overlapped with 6 cloned or mapped genes related to rice blast resistance, and the remaining 19 loci were new loci. In addition, the analysis of the rice subpopulations showed that the average resistance level of tropical japonica subpopulation was the highest, and the lowest was the temperate japonica subpopulation. These findings provided DNA markers for molecular-assisted breeding of blast resistance and genomic location information for subsequent cloning of these genes..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 163 (2021)
Distribution Characteristics of the HLA-DRB1 Allele and Anti-HLA-DRB1 Antibodies in Uremia Patients
GUO Xuli, ZHU Quan, WANG Huipeng, XIE Min, and LUO Qizhi
In order to study the frequency distribution of HLA-DRB1 alleles and anti-HLA-DRB1 antibody types in patients with uremia in southern China, the peripheral blood of uremia patients and healthy controls (HCs) was collected, and HLA-DRB1 allele was genotyped via polymerase chain reaction-sequence specific oligonucleotideIn order to study the frequency distribution of HLA-DRB1 alleles and anti-HLA-DRB1 antibody types in patients with uremia in southern China, the peripheral blood of uremia patients and healthy controls (HCs) was collected, and HLA-DRB1 allele was genotyped via polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). A Luminex system was used to detect the types and frequencies of anti-HLA-DRB1 antibodies in patients undergoing renal transplantation due to uremia. HLAMatchmaker database was used to compare and analyze the epitopes of HLA-DRB1 and their amino-acid sequences. The types and frequencies of HLA alleles and anti-HLA antibodies in patients were analyzed by statistical software. It was found that the frequency of the HLA-DRB1*13 allele in uremia patients was significantly lower than that of HCs (4.33% vs. 13.37%, P<0.001), the frequency of the HLA-DRB1*14 allele was significantly higher than that of HCs (12.01% vs. 4.12%, P<0.001). Among all types of anti-HLA-DRB1 antibodies, the frequency of anti-HLA-DRB1*01/*13/*14 antibodies was significantly lower than that of other anti-HLA-DRB1 antibodies (P<0.05). A common epitope 77T in HLA-DRB1*01/*13/*14 alleles was discovered using HLAMatchmaker database. These indicate that in terms of gene frequency, HLA-DRB1*13 is correlated negatively with uremia prevalence and may have protective effects on individuals. HLA-DRB1*14 is correlated positively with uremia prevalence, and may be a susceptible factor of uremia. Levels of anti-HLA-DRB1*01/ *13/*14 antibodies in uremia patients were significantly lower than those of other anti-HLA-DRB1 antibodies, probably due to the presence of a common epitope 77T. This study will lay the foundation for an in-depth understanding of the relationship between HLA-DRB1 gene and uremia, and provide experimental evidence for elucidating the role of HLA-DRB1 gene in uremia and kidney transplantation..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 170 (2021)
Overexpression of TNFAIP1 Promote the Inhibitory Effect of Temozolomide on Migration and Invasion of Glioma Cells U251
XU Feiyu, CHEN Guo, LONG Shengwen, and WEI Chenxi
In order to further study the mechanism of chemotherapy resistance in human gliomas and explore potential therapeutic methods, we investigated the effects of tumor necrosis factor α-induced protein 1 (TNFAIP1) on migration and invasion. Reverse transcription PCR and Western blot assays showed that temozolomide increaseIn order to further study the mechanism of chemotherapy resistance in human gliomas and explore potential therapeutic methods, we investigated the effects of tumor necrosis factor α-induced protein 1 (TNFAIP1) on migration and invasion. Reverse transcription PCR and Western blot assays showed that temozolomide increased the expression of TNFAIP1. We overexpressed TNFAIP1 in U251 cells, then we found it could promote the inhibition of temozolomide (TMZ) on migration and invasion of U251 cells, and this effect was achieved by inhibiting epithelial-to-mesenchymal transition (EMT) signaling pathway. This finding is helpful to provide a new theoretical basis for clinical treatment of human glioma and provide a new strategy for its treatment..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 178 (2021)
Analysis and Significance of EGLN1 Expression in Clear Cell Renal Cell Carcinoma Based on Bioinformatics
YAO Yutong, LIU Yan, DAI Lu, and XIA Binbin
In order to explore the expression and clinical significance of egl-9 family hypoxia-inducible factor 1 (EGLN1) in clear cell renal cell carcinoma (ccRCC), based on the gene expression data of ccRCC patients in the TCGA database to analyze EGLN1 in ccRCC tissues and normal kidney tissues, the difference in expression oIn order to explore the expression and clinical significance of egl-9 family hypoxia-inducible factor 1 (EGLN1) in clear cell renal cell carcinoma (ccRCC), based on the gene expression data of ccRCC patients in the TCGA database to analyze EGLN1 in ccRCC tissues and normal kidney tissues, the difference in expression of EGLN1 in ccRCC was analyzed using the UALCAN platform. Based on the ccRCC clinical data in the TCGA database, the survival analysis of the EGLN1 high and low expression group, the Cox analysis of the correlation between EGLN1 expression and overall survival, and the gene enrichment analysis of the EGLN1 gene expression in the clinical data were performed. The possible potential interactions between encoded proteins were searched through the STRING database, and a protein interaction network was constructed to express them, obtain EGLN1 related proteins, and perform related analysis on the GEPIA platform. Analysis of the sample expression data in the TCGA database shows that the expression of EGLN1 in ccRCC is higher than that in normal kidney tissue. The analysis of the UALCAN platform found that the high expression of EGLN1 is correlated with the age of the patient. Survival analysis showed that ccRCC patients had a better prognosis when EGLN1 was overexpressed. Univariate and multivariate Cox analysis indicated that the expression of EGLN1 is an independent risk factor for the survival of ccRCC patients. Gene enrichment analysis suggests that the expression of EGLN1 gene is closely related to RCC pathway, ubiquitin-mediated proteolysis pathway, and apoptosis pathway. The results show that EGLN1 may be a molecular marker for evaluating the prognosis of ccRCC..
Acta Laser Biology Sinica
- Publication Date: Sep. 01, 2021
- Vol. 30, Issue 2, 185 (2021)