In order to realize the effective integration of the digital construction of experimental teaching and the cultivation of top innovative talents in biology, and solve the problems of the traditional experiment of the subcellular localization observation of plant protein, such as high experiment cost, insufficient number of precision instrument sets and insufficient practical ability of students, the virtual simulation technology is used to develop the virtual simulation experiment of subcellular localization observation of plant protein in Chinese and English bilingual teaching. This virtual experimental teaching resource covers the preparation of Arabidopsis protoplast, protoplast transformation mediated by polyethylene glycol, fluorescence microscope observation and analysis of fluorescent protein localization and other experimental contents, creating a new experimental teaching model that combines scene, immersion and interaction. Through its application in undergraduate experimental teaching, it deepens students’ understanding of the protein function research experimental technology, improves the curriculum system of the general biology experiment, realizes the integration of scientific research practical training and experimental teaching, and cultivates students’ innovative ability, scientific research accomplishment and international competitiveness, which is an effective supplement to the traditional experimental teaching.