Non-diffracting beams have attracted attention in recent years due to their unique properties, such as diffraction-free propagation and self-repairing and self-accelerating ability, which make them promising candidates for microscopic imaging applications. Non-diffraction beams can suppress beam diffraction during propagation, thereby improving the imaging resolution. Moreover, their self-healing characteristic facilitates quick wave-front recovery after passing through a strongly scattering medium, enhancing imaging depth and signal-to-noise ratio. Their self-acceleration feature expands the detection dimension of light field information, enabling multi-dimensional reconstruction imaging. Based on the characteristics of several biological micro-imaging technologies, this paper discusses the application and research progress of non-diffracting beams, specifically Bessel and Airy beams, in high-resolution biological micro-imaging.