The Yb₂O₃ component was introduced into the Er³⁺/Ce³⁺ co-doped tellurite glasses with the composition of TeO₂-WO₃-Na₂O-Nb₂O₅ to study the effect of Yb³⁺ on the 1.53 μm spectroscopic properties of Er³⁺. The X-ray diffraction (XRD) curve and Raman spectrum were measured to investigate the structure nature of synthesized tellurite glasses. The absorption spectrum, upconversion emission spectrum and fluorescence spectrum were measured to evaluate the improved effect of Yb³⁺ concentration on the 1.53 μm band fluorescence of Er³⁺. Results of the measured 1.53 μm band fluorescence intensity show a significant improvement with the increase of Yb³⁺ concentration, while the total quantum efficiency reveals a similar increasing trend. The results of the present work indicate that Er³⁺/Ce³⁺/Yb³⁺ tri-doped tellurite glass has good prospect as a promising gain medium applied for the 1.53 μm broadband amplifier.