In order to obtain the high quality all-in-focus imaging in large depth of view, an all-in-focus image reconstruction technology based on single exposure light field imaging and guided filter was proposed. First, the field of view information was collected by the optical field imaging, and the multi-focus image source set was obtained by the optical field reconstruction. Then the guided filter method was used to determine the weight of image fusion at all levels, and finally the image fusion was performed to obtain the all-in-focus image with extremely large depth of field. The experimental results show that the proposed method not only effectively ensures the background consistency, but also keeps the edge retention to reconstruct the all-in-focus image with high quality. Therefore, the proposed technology can be potentially adopted in many applications such as on-site monitoring, geographical exploration, military reconnaissance and unmanned technologies, etc.