To evaluate the evolution of response of solar blind ultraviolet (SBUV) intensified CCD (ICCD) versus working time, a novel fatigue property testing scheme is proposed and actual experiments are carried out for two typical work patterns. At first, two typical working modes for SBUV-ICCD are introduced based on the fundamental construction and principle. Secondly, the device fatigue property is defined and a novel fatigue testing scheme is designed. Finally, fatigue testing for two SBUV-ICCDs is done and their uncertainties are analyzed. The testing results show that the fatigue properties of a homemade SBUV-ICCD in photoelectric simulation mode and photo counting mode are 13% and 3%, respectively, while the corresponding parameters of an abroad SBUV-ICCD are better than 1%, and the scheme′s uncertainty is 1%.