A Fabry-Perot micro-cavity is fabricated by on-line fiber cutting-welding method. The asymmetrical fiber Fabry-Perot micro-cavity is designed and produced by cutting a standard single-mode fiber and welding the fiber end with the core-offset structure. The length of the Fabry-Perot micro-cavity could be controlled within a certain range of accuracy based on the on-line fiber cutting-welding method. According to this method, a micro-machined Fabry-Perot micro- cavity with a length of about 147 μm is achieved and its spectral characteristic is also investigated in our experiment. This proposed method is suitable to produce a micro-fiber-optic structure with improved and controlled precision, which is attractive for the fiber processing field. Moreover, the fabricated Fabry-Perot micro-cavity also has potential application in the microfluidic system and biochemical detection area.