Aiming at the issue that image-resolution of the electro-optical imaging system can be depressed by the image jitter, a ration analysis method of the requirement of jitter was proposed. The jitter was classified into high frequency jitter and low frequency jitter, the high frequency jitter divides into Gauss, sine and linear, the low frequency jitter was defined as linear jitter, the modulation transfer function of them was given, and the modulation transfer function of the whole system was built. Based on the resolution equal notion and the modulation transfer function model, the ration analysis method of the jitter was given, the analysis flow and arithmetic was also presented. At last, the method was simulated through computer based on specially appointed system parameter and the analysis flow. The simulation results indicate that the low frequency linear jitter tolerance is max, the high linear jitter tolerance is next, the high frequency Gauss jitter tolerance and the high frequency sine jitter tolerance is min.