Laser multilayer deposition of Rene88DT superalloy on DD3 single-crystal substrate is conducted. The influences of the crystal orientation of the substrate and the profile of the solid/liquid interface of the molten pool on the deposited microstructure are investigated. A unique strategy is proposed by adjusting the angle between the substrate surface and the substrate crystal orientation. This approach prevents the formation of the turned dendrite, thus obtaining a fully directional microstructure in the deposits.