In order to study the relationship between the macro-bending loss characteristics of the single-mode fiber and the temperature change, the thermo-optical effect and thermal expansion effect are corrected according to the theoretical formula proposed by Faustini L. Based on this formula, the macro-bending loss of SMF28 and 1060XP single-mode fiber with core-cladding-infinite coating structure under the influence of temperature is simulated and analyzed. The influence of temperature, wavelength, bending radius and other factors on the macro-bending loss of SMF28 single-mode fiber is explored, and the linear fitting results between the temperature and the two kinds of single-mode fibers are obtained. The results show that the macro-bending loss increases with the decrease of bending radius, and the increase of wavelength. The loss also decreases with the increase of temperature. Under certain bending radius and wavelength, the macro-bending loss and temperature are in monotonic relationship, which means increasing the number of bending turns can effectively increase the sensitivity to temperature.