A satellite borne Solar Backscatter Ultraviolet Spectrometer (SBUS) developed by our country has been put into operation in-orbit for the first time. This paper introduces the composition of the SBUS and gives its main working modes(atmospherical mode, solar modes and standard lamp mode). After analysis of run missions, a reasonable scheme of operation control in-orbit is presented according to the characteristics of various operation parameters and complicated mode transitions, and then the main points of software design is explained in detail. The remote sensing data of ultraviolet radiation measured in-orbit with the solar/atmospheric working modes are given, which shows that the wavelength reproducibility by the discrete solar mode is ±0.03 nm and the dynamic range of the system reaches 106 magnitude with the function of automatic gain control. Obtained results demonstrate that the proposed measurement modes have been implemented correctly and the scheme of operation control is completed. The SBUS are working in a best state with reasonable paremeters and optimum performance.