Effects of Low-Temperature Stress on Grain Seeds Germination by Infrared Spectroscopy

Qian Che; Gang Liu; Xue Tian; Quanhong Ou; Weimin Yan; Youming Shi

doi:10.3788/LOP221586

Journals >Laser & Optoelectronics Progress >Volume 60 >Issue 15 >Page 1530003 > Article

Laser & Optoelectronics Progress
Vol. 60, Issue 15, 1530003 (2023)

Effects of Low-Temperature Stress on Grain Seeds Germination by Infrared Spectroscopy

Qian Che¹, Gang Liu^1,*, Xue Tian¹, Quanhong Ou¹..., Weimin Yan¹ and Youming Shi²|Show fewer author(s)

Author Affiliations

¹School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500, Yunnan, China

²School of Physics and Electronic Engineering, Qujing Normal University, Qujing 655011, Yunnan, China

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DOI: 10.3788/LOP221586 Cite this Article Set citation alerts

Qian Che, Gang Liu, Xue Tian, Quanhong Ou, Weimin Yan, Youming Shi. Effects of Low-Temperature Stress on Grain Seeds Germination by Infrared Spectroscopy[J]. Laser & Optoelectronics Progress, 2023, 60(15): 1530003 Copy Citation Text

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Fig. 1. Fourier transform infrared spectra of grain seeds germinated at different temperatures. (a) Quinoa; (b) highland barley; (c) rice; (d) wheat

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Fig. 2. Curve fitting spectra of quinoa seeds at different germination temperatures in the range of 1200-950 cm^-1

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Fig. 3. Curve fitting spectra of quinoa seeds at different germination temperatures in the range of 1700-1600 cm^-1

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Grain varieties	Temperature
Grain varieties	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
Quinoa	8±0.9	4±0.5	5±0.4	2±0.1	2±0.01
Highland barley	52±3.6	52±3.1	40±2.6	22±1.8	18±1.5
Rice	18±1.3	0	0	0	0
Wheat	80±2.8	70±3.1	64±3.1	8±0.7	0

Table 1. Effects of low-temperature stress on germination potential of four grain seeds

Grain varieties	Temperature
Grain varieties	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
Quinoa	22±1.3	9±0.9	8±0.7	6±0.4	4±0.3
Highland barley	96±3.9	92±4.3	86±2.3	78±2.8	74±3.0
Rice	78±3.1	68±2.8	0	0	0
Wheat	96±3.3	88±3.4	84±3.1	80±2.7	8±0.6

Table 2. Effects of low-temperature stress on the seed germination rate of fourgrain seeds

Grain varieties	Temperature
Grain varieties	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
Quinoa	12.56±1.00	5.63±0.02	5.32±0.04	5.24±0.03	1.67±0.01
Highland barley	18.57±1.41	18.72±0.05	22.05±1.24	14.59±1.35	16.66±0.45
Rice	2.85±0.05	0.18±0.02	0	0	0
Wheat	33.36±0.71	32.24±1.48	24.78±1.20	11.81±0.75	10.52±0.41

Table 3. Effects of low-temperature stress on germination index of fourgrain seeds

Wave number ν /cm^-1	Temperature
Wave number ν /cm^-1	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
998	25.91	26.53	27.07	29.21	28.34
1023	5.62	13.36	10.94	6.94	9.09
1047	27.43	22.02	22.98	24.40	23.15
1080	5.37	6.61	6.01	5.88	5.89
1100	14.05	15.54	14.65	14.20	14.39
1124	2.85	1.96	2.04	2.18	1.80
1154	13.42	14.93	14.44	13.84	13.89
Total	94.65	100.50	98.13	96.65	96.55

Table 4. Sub-peak positions and areas of quinoa seeds at different germination temperatures in the range of 1200-950 cm^-1

Wave number ν /cm^-1	Temperature
Wave number ν /cm^-1	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
993	21.70	23.18	22.43	22.83	24.05
1024	29.38	24.77	26.47	21.08	18.48
1054	12.18	15.49	13.75	15.69	17.52
1080	5.31	4.80	5.00	4.39	4.27
1102	19.06	22.22	20.52	22.12	21.90
1126	0.79	0.68	0.69	0.57	0.71
1158	14.82	13.91	14.15	12.85	13.34
Total	103.24	105.05	103.01	99.53	100.27

Table 5. Sub-peak positions and areas of highland barley seeds at different germination temperatures in the range of 1200-950 cm^-1

Wave number ν /cm^-1	Temperature
Wave number ν /cm^-1	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
998	27.49	19.89	17.76	20.75	23.82
1023	11.68	16.36	24.44	21.89	14.83
1050	19.01	11.18	9.18	11.27	17.08
1080	5.27	3.88	4.13	4.44	4.06
1102	16.86	16.41	17.93	18.75	19.32
1126	1.05	0.60	0.59	0.62	0.70
1156	13.47	11.53	12.84	13.73	13.12
Total	94.83	79.85	86.87	91.45	92.93

Table 6. Sub-peak positions and areas of rice seeds at different germination temperatures in the range of 1200-950 cm^-1

Wave number ν /cm^-1	Temperature
Wave number ν /cm^-1	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
991	19.73	21.55	24.34	23.61	23.44
1023	30.29	26.59	21.31	22.27	18.70
1054	10.62	13.82	16.17	14.15	16.17
1080	4.27	4.44	4.00	4.22	3.99
1101	22.86	24.13	26.19	22.92	23.27
1126	0.47	0.47	0.47	0.55	0.47
1159	13.59	13.20	12.73	13.71	12.60
Total	101.83	104.20	105.21	101.13	98.64

Table 7. Sub-peak positions and areas of wheat seeds at different germination temperatures in the range of 1200-950 cm^-1

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	4.36	3.69	3.62	4.89	4.95
α-helix	3.40	3.13	3.09	2.79	2.41
Random coil	0.89	0.78	1.76	0.28	2.59
β-turns	3.33	2.85	2.97	3.67	0.90
Total	11.98	10.45	11.44	11.63	10.85

Table 8. Secondary structure peak areas of quinoa seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	36.37	35.41	31.71	42.05	22.76
α-helix	28.38	29.95	27.00	24.02	30.68
Random coil	7.44	7.42	15.40	2.38	20.65
β-turns	27.81	27.22	25.94	31.65	25.92

Table 9. Percentage of secondary structure peak areas of quinoa seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	3.09	5.16	3.10	3.07	3.36
α-helix	2.89	1.99	3.05	2.02	4.55
Random coil	2.13	1.40	0.49	0.95	0.32
β-turns	2.57	2.95	2.76	3.07	2.75
Total	10.68	11.50	9.40	9.11	10.98

Table 10. Secondary structure peak areas of highland barley seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	28.91	35.02	32.94	33.71	30.65
α-helix	27.06	20.36	32.44	22.14	41.35
Random coil	19.95	14.31	5.26	10.47	2.95
β-turns	24.07	30.30	29.35	33.69	25.04

Table 11. Percentage of secondary structure peak areas of highland barley seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	3.54	4.02	3.97	4.60	3.50
α-helix	3.70	3.37	4.05	5.07	4.28
Random coil	1.79	1.47	1.66	1.32	3.02
β-turns	2.56	3.33	3.20	4.16	2.35
Total	11.59	12.19	12.88	15.15	13.15

Table 12. Secondary structure peak areas of rice seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	30.61	32.95	30.82	30.39	26.56
α-helix	31.89	27.65	31.42	33.46	32.57
Random coil	15.43	12.05	12.90	8.73	23.00
β-turns	22.07	27.36	24.86	27.42	17.85

Table 13. Percentage of secondary structure peak areas of rice seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	5.29	4.31	5.27	5.52	5.64
α-helix	4.52	6.63	7.23	7.06	7.05
Random coil	0.88	0.09	0.22	0.18	0.31
β-turns	5.11	3.59	4.30	4.62	4.74
Total	15.80	14.62	17.02	17.38	17.74

Table 14. Secondary structure peak areas of wheat seeds in the range of 1700-1600 cm^-1 at different germination temperatures

Secondary structure	Temperature
Secondary structure	25 ℃（CK）	20 ℃	15 ℃	10 ℃	4 ℃
β-sheet	33.47	29.50	31.00	31.78	31.76
α-helix	28.60	45.30	42.45	40.63	39.77
Random coil	5.56	0.62	1.30	1.01	1.77
β-turns	32.36	24.58	25.25	26.57	26.69

Table 15. Percentage of secondary structure peak areas of wheat seeds in the range of 1700~1600 cm^-1 at different germination temperatures

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