Single-layer, double-layer and multi-layer coating lycopene nanocapsules are prepared by the micro-emulsion technology and composite reunion method, and the effect of coating materials on particle size and its distribution is analyzed by dynamic light scattering method. The studied results show that the more serious aggregation can be produced in phosphate buffer of pH=6.5 for the single-layer lycopene nanocapsules coated by alkali-treated gelatin. Compared with the single-layer coating lycopene nanocapsules, the weaker aggregation can be observed in phosphate buffer of pH=5.5 for the double-layer lycopene nanocapsules coated by alkali-treated gelatin and k-carrageenan. However, the multi-layer coating lycopene nanocapsules can be dispersed in stability in phosphate buffer of pH=5.5 and there is no aggregation.