Alzheimer’s disease (AD) is a typical neurodegenerative disease. $\beta$-amyloid (A$\beta )$ plaque is the most prominent pathological biomarker associated with the progression of AD. Conventional A$\beta$ probes, including commercial probe ThT, usually suffer from tedious washing procedures. Herein, novel AIE-active A$\beta$ probes with excellent water solubility, named DE-V1-PYC3 and DE-V1-PYOH, were developed for the detection and image of A$\beta$ without tedious washing procedures. Compared with commercial probe ThT, the AIE-active A$\beta$ probes exhibited better sensitivity and affinity to A$\beta$ aggregates. Moreover, for ThT, the washing procedures are essential to obtain high signal-to-noise ratio (SNR) images of A$\beta$ plaques in AD brain tissue slices. DE-V1-PYC3 and DE-V1-PYOH can label A$\beta$ plaques in AD brain tissue slices with high SNR even without tedious washing procedures.