The surface plasmon resonance (SPR) sensor is widely used in biology, medicine, chemistry, drug screening, environmental monitoring, and food safety. However, the SPR sensor system can be easily affected by device self noise of optical source and photoelectricity detector etc, and ambient lights noise during detections, where the ambient light noise is particularly obvious. So the operation of SPR sensor needs to be in a shading environment, which is not conducive for rapid detections. To solve this problem, the characteristics of ambient light noise are analyzed on the basis of self-developed angle scanning surface plasmon resonance sensor. A data processing method, which combines an arithmetic average filtering and a low- pass filtering, is innovatively put forward to suppress the noise under fluorescent lights illumination. The experimental results of phosphate buffered saline (PBS) buffer detection shows that the proposed method can offer accurate detection under fluorescent lights illumination. The error of measurement results is 0.0027% , relative to the results in a shading measurement. The proposed method can effectively enhance the anti interference ability to ambient light of the angle scanning SPR sensor. Moreover, it has good application and popularization value.网络出版日期<2015-04-29>