• Journal of Innovative Optical Health Sciences
  • Vol. 18, Issue 1, 2450026 (2025)
Yujun Tang1,2,3, Linbo Wang2, Gang Wen2,*, and Hui Li2,3,**
Author Affiliations
  • 1School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Suzhou 230041, P. R. China
  • 2Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China
  • 3Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201 P. R. China
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    DOI: 10.1142/S1793545824500263 Cite this Article
    Yujun Tang, Linbo Wang, Gang Wen, Hui Li. Quantitative evaluation of intensity fidelity of super-resolution reconstruction for structured illumination microscopy[J]. Journal of Innovative Optical Health Sciences, 2025, 18(1): 2450026 Copy Citation Text show less

    Abstract

    Super-resolution structured illumination microscopy (SR-SIM) relies heavily on post-processing reconstruction to obtain high-quality SR images from raw data. Although many SIM reconstruction algorithms have been developed to recover fine cellular structures with high fidelity even from the noisy data, whether the pixel intensities of reconstructed SR images are still proportional to the original fluorescence intensity has been less explored. The linearity between the intensity before and after reconstruction is defined as the intensity fidelity. Here, we proposed a method to evaluate the reconstructed SR image intensity fidelity at different spatial frequencies. With the proposed metric, we systematically investigated the impact of the key factors on the intensity fidelity in the standard Wiener-SIM reconstructions with simulated data, then evaluated the intensity fidelity of the SR images reconstructed by representative open-source packages. Our work provides a reference for SR-SIM image intensity fidelity improvement.
    Yujun Tang, Linbo Wang, Gang Wen, Hui Li. Quantitative evaluation of intensity fidelity of super-resolution reconstruction for structured illumination microscopy[J]. Journal of Innovative Optical Health Sciences, 2025, 18(1): 2450026
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