A time resolved transient absorption spectroscopy was performed to investigate the photoprotection mechanism in light harvesting complex II of broypsis corticulans. A 667 nm femotosenond laser and white light pulse were used as pump and probe lights. The range and accuracy of the delay line between probe and pump were 340 ps and 134 fs, respectively. The experiment results show that the absorption of the probe is dynamically changing after pump light exciting. The results of the multi-exponential fitting, fluorescence emission and excitation spectra show that the transient absorption spectra from 500 nm to 600 nm mainly originate from carotenoids and the aggregated light harvesting complex II contains at least four types of carotenoids functioning photoprotection which have S0→Sn transition spectral properties 511 nm and 554 nm, 522 nm and 541 nm, 530 nm and 563 nm (corresponding to siphonaxanthin), 536 nm and 575 nm. Carotenoids play the photoprotection role not only in a direct manner by quenching the triplet state of chlorophyll a in several picoseconds, but also in an indirect manner by quenching the singlet state of oxygen in several hundreds of picoseconds.