A calibration equipment for the range static explosion experiment site is designed, a calibration method for high speed camera based on genetic simulated annealing is studied, and an experiment which calibrates the internal and external parameters of the binocular high speed camera by the designed calibration equipment is carried out. According to the circular manually-labelled position parameters collected in experiment, the parameters of the binocular high-speed camera are calibrated by the genetic simulated annealing based method and the Tsai two step calibration method respectively. The parameters calibrated by the two methods are used to restore the spatial coordinates of known points in sight. By comparing the restored results with the measurement coordinates, it is found that the maximum deviation of the spatial coordinates restored by the proposed method is 0.0082 m, which is better than the maximum deviation of the Tsai two-step calibration method (0.0201 m). With the proposed method, the calibration accuracy of the high speed camera is improved, and the method is of great significance for the improvement of the fragment velocity measurement accuracy.