Synchronous fluorescence spectra of different plasma solution concentrations in the UV wavelength are investigated using three-dimensional synchronous fluorescence spectroscopy. The results show that there are three main excitation peaks of plasma proteins(near 257 nm, 274 nm and 280 nm),and the peak values change with different plasma solution concentrations. By changing the offset (that is Δλ) value of the synchronous fluorescence spectra obtained, there are three synchronous fluorescence peaks of plasma, of which the values are 90 nm, 72 nm and 44 nm. According to the experimental data, it shows that the intrinsic fluorescence of the plasma in all groups exists the fluorescence resonance energy transfer and the second light absorption.