Digital micromirror device is added into the fluorescence confocal microscopy system,insteads of lighting pinhole of a traditional confocal microscopy system.The modulation properties of digital micromirror device divide a light source into multiple beams through loading different pictures on digital micromirror device.Four-channel,six-channel and nine-channel fluorescence images of photo cells were detected and the system resolution was analyzed by measuring the depth response curve.The experimental results show that the microscopy system transfers from point-to-point imaging into the parallel confocal microscopy imaging based on digital micromirror device.The system provides a fast scanning speed and a high resolution.