• Optical Instruments
  • Vol. 42, Issue 4, 67 (2020)
Tongda WEI1, Yunhai ZHANG1,*, Jian CHANG1, Lin JI1..., Haomin YANG1 and Jia MIAO2|Show fewer author(s)
Author Affiliations
  • 1Key Laboratory of medical optics, Suzhou Institute of biomedical engineering and technology, Chinese Academy of Sciences, Suzhou 215163, China
  • 2Suzhou Branch,Jiangsu Institute of Medical Devices Testing, Suzhou 215163, China
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    DOI: 10.3969/j.issn.1005-5630.2020.04.011 Cite this Article
    Tongda WEI, Yunhai ZHANG, Jian CHANG, Lin JI, Haomin YANG, Jia MIAO. Two-photon stimulated emission depletion composite microscopy[J]. Optical Instruments, 2020, 42(4): 67 Copy Citation Text show less
    Optical system for 2P STED composite microscope
    Fig. 1. Optical system for 2P STED composite microscope
    Side view of optical layout in the microscope
    Fig. 2. Side view of optical layout in the microscope
    Point spread function scanning imaging in XY plane
    Fig. 3. Point spread function scanning imaging in XY plane
    2P imaging of fluorescent microspheres with different depth
    Fig. 4. 2P imaging of fluorescent microspheres with different depth
    Resolution measurement by fluorescence microsphere imaging
    Fig. 5. Resolution measurement by fluorescence microsphere imaging
    2P STED composite microscope prototype
    Fig. 6. 2P STED composite microscope prototype
    Fluorescence in-situ hybridization (FISH) imaging
    Fig. 7. Fluorescence in-situ hybridization (FISH) imaging
    2P imaging of brain.
    Fig. 8. 2P imaging of brain.
    Second harmonic imaging of skin.
    Fig. 9. Second harmonic imaging of skin.
    2P depth imaging
    Fig. 10. 2P depth imaging
    Super resolution imaging comparison of fluorescent microsphere
    Fig. 11. Super resolution imaging comparison of fluorescent microsphere
    Tongda WEI, Yunhai ZHANG, Jian CHANG, Lin JI, Haomin YANG, Jia MIAO. Two-photon stimulated emission depletion composite microscopy[J]. Optical Instruments, 2020, 42(4): 67
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