To ensure the frequency accuracy of a heterodyne laser source in the ambient temperature range of -20°C to 40°C, a dual-longitudinal-mode thermally stabilized He–Ne laser based on non-equilibrium power locking was designed. The ambient adaptive preheating temperature setting scheme ensured the laser could operate normally in the range of -20°C to 40°C. The non-equilibrium power-locked frequency stabilization scheme compensated for the frequency drift caused by different stabilization temperatures. The experimental results indicated that the frequency accuracy of the laser designed in this study could reach 5.2 × 10^-9 in the range of -20°C to 40°C.