System-level polarized calibration is an important link in the development of directional polarization camera(DPC), which is of great significance for improving quantitative detection applications such as atmospheric aerosols and cloud phases. The polarization response calibration model is established combining with matrix optics and radiation theories, and key impact parameters are calibrated. An integrating sphere reference light source with large aperture and sectional viewing field measurement is used to eliminate the influence of the optical wedge plate on the non-consistency of the field view of DPC three partial channel, and high precision measurements of the high and low frequency relative transmittances are achieved. By using the Fourier series analysis method, the measurement model of the polarization degree of full view is established, and the measurement error introduced by the polarization azimuth absolute position of the reference light source is eliminated, which guarantees the precise measurement of polarization degree. The variable polarization light source and integrating sphere radiation source with large aperture are used for the comparison and precision analysis, the DPC full view polarization calibration uncertainty is better than 0.5%, and the DPC measured polarization degree uncertainty under natural light is better than 0.05%, which confirms the precision and reasonability of polarization remote sensor with wide field of view polarization radiation response calibration model. This system-level polarized calibration method can meet the requirements of high-precision polarization observation scientific application of wide field optical polarization remote sensor.