Aiming at the problem of speech distortion caused by channel difference, based on the methods of probability statistics and image comparison, the auditory and acoustic analysis of the audio recorded by several recording tools in the laboratory environment are carried out. By extracting the characteristic maps and data of different speakers in different channels, the influence of channel differences on the essential and non-essential differences of speech is discussed. The results show that the channel difference will lead to some non-essential differences between the recorded speech signals. Even if the same kind of channel, when there are differences between recording equipments, the same will lead to differences.