Turbulent kinetic energy dissipation rate (turbulent dissipation rate) is an important parameter of the turbulence. There is a new measurement on the turbulence, which estimats the turbulent dissipation rate using the spectral width of wind profiler. Based on studying the influencing factor on the spectral width of echo signal of airdar16000 wind profiler radar, and extracting the spectral width contributing by turbulence, the turbulent dissipation rate can be estimated. Results show that the value of spectral width from turbulence is below 0.5 m[EQUATION]s[EQUATION], and the oblique beams are more reliable than the vertical beam, and the turbulent dissipation rate distributes between 10[EQUATION]m[EQUATION]s[EQUATION] and 10[EQUATION]m[EQUATION]s[EQUATION]. It decreases with the increase of the height. The result accords with the theory, so it is feasible to estimate the dissipation rate from wind profiler radar.