Bangchao Xi, Jiayu Yang, Shaolei Huang, Haozheng Dai, Juntian Zeng, Dongxu Zhang. A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection[J]. Laser & Optoelectronics Progress, 2023, 60(17): 1722001
- Laser & Optoelectronics Progress
- Vol. 60, Issue 17, 1722001 (2023)
Fig. 1. Schematic diagram of the orthogonal optical path structure
Fig. 2. Schematic diagram of light passing through optical components
Fig. 3. Schematic diagram of multi-channel integration and switching device
Fig. 4. Rapid amplification supporting device. (a) Microfluidic chip; (b) amplification heating light path; (c) optical fiber with linear spot; (d) physical map of multi-channel integration and switching device
Fig. 5. Block diagram of the hardware structure of the detection system
Fig. 6. Light Tools simulation. (a) Optical path structure; (b) spot size
Fig. 7. Test environment and conditions. (a) Fully automatic nucleic acid detection device; (b) internal structure diagram
Fig. 8. Resolution comparison between the straight-axis multi-channel optical detection systems and commercial PCR instruments.(a) FAM; (b) HEX; (c) ROX
Fig. 9. Channel crosstalk data histogram
Fig. 10. Detection curves of straight-axis multi-channel optical detection systems. (a) Random result 1; (b) random result 2; (c) random result 3; (d) random result 4
|
Table 1. Parameters of LED
|
Table 2. Parameters of optical path distance
| ||||||||||||||||||||||||||||||
Table 3. Fluorescent dye detection repeatability test results
Set citation alerts for the article
Please enter your email address
© Copyright 2018-2021 | Chinese Laser Press. All Rights Reserved 沪ICP备15018463号-20