The stone cultural relics are important cultural heritage, however, fissures can lead to destabilization, water seepage and weathering diseases. Therefore, it is of great significance to develop a suitable grouting material for repairing fissures in stone cultural relics. The grouting material was prepared by adding graphene oxide (GO) into natural hydraulic lime (NHL2). The influence of GO content on hydration process and products of NHL2 was studied using SEM, TG, XRD and FT-IR. The feasibility of this material for grouting reinforcement of stone cultural relics was evaluated by mechanical properties and simulated grouting tests. The results indicate that the addition of GO can accelerate hydration process and promote formation of regular and dense flower-like aggregated hydration products. When GO content is 0. 05% ( mass fraction), the sample has the highest content of hydration products and the best mechanical properties. The compressive strength and flexural strength of samples cured for 28 d can reach 2. 83 and 1. 63 MPa, which increases by 19. 91% and 23. 40% with the blank sample, respectively. Furthermore, there is excellent bonding strength between grouting material and sandstone, the interface of the two is closely combined with good compatibility. This work provides reference for grouting reinforcement of fissures in stone cultural relics.