Although the traditional fluorescence spectroscopy method can be used to evaluate the physiological status of plants, there are some measured defects, such as weak anti-jamming, large amount of data in plant remote sensing detection. Aiming at this matter, a fluorescence lifetime imaging technique is presented. The plant fluorescence lifetime and spectral information are combined to analyze the plant physiological information, and a wide spread of plant physiological status can be measured accurately. The many same fluorescence signal can be produced, while the plant is irradiated by continuous laser pulse in the time resolution measurement method. At the same time, a complete fluorescent signal will be got, while start-up delay time is changed constantly. Then, the plant fluorescence life can be retrieved by deconvolution arithmetic. The spectral information and fluorescence lifetime of living Viburnum awabuki plants was studied. Then, the results show that the plant fluorescence lifetime is linear with chlorophyll concentration. The linear correlation coefficient reaches into 0.9051 in specific range of 0.03~0.06 mg/cm2, and plant fluorescence lifetime is a better description of chlorophyll concentration than the fluorescence spectroscopy in the range.