3D displacement and velocity were measurd by using a single frame fringe which is recorded by an interlaced scanning camera for a moving object. One frame deformed fringe is operated by dividing into two single field fringes, reconstructing 3D surface shapes by directly using Fourier transform profilometry, extracting 2D binarization templates from the modulation of single fields, computing the centroid to get sub-pixel locating point, and processing the shape data by bi-cubic interpolation and calibration, it can accomplish the measurements of 3D displacement and 3D average velocity within the interval of a field period time. In the 3D space experiment with constant velocity of a 3D object, the maximum absolute error of measured velocity is 0.6mm/s, and the relative error is 0.57%. This method needs only one frame deformed fringe to measure 3D displacement and velocity, and improves temporal resolution and measuring precision.