The self-developed portable angle scanning surface plasmon resonance biosensor is used in the quantitative detection of low concentration solution, because the existing quantitative detection methods are complicated and with low resolution. The validity of this method and the developed device is demonstrated by experiments. The quantitative detection is proceeded in glucose and glycine solutions. The detection results show that the direct detection of glucose solution distinguish the concentration of 0.1 mg/ml. The quantitative detection method of low concentration solution based on the surface plasmon resonance is simple. The developed device is portable and easy to operate. The rapid and real-time detection with high resolution can be realized by this method.