Aiming at the problem that fiber loss and nonlinear effect will degrade the bending performance of optical fiber, a refractive index profile structure G.654.E optical fiber with ditch layer, transition layer and appropriate cladding ratio is designed, and the effects of waveguide structure of G.654.E optical fiber on attenuation and optical performance are studied. The test results show that at 1550 nm and 1625 nm wavelength, the typical attenuation values of optical fiber are 0.162 dB/km and 0.178 dB/km respectively. When the bending radius is 10 mm and one turn is wound, the macro bending loss is lower than 0.04 dB and 0.06 dB respectively. When the bending radius is 30 mm and 100 turns, the typical values of macro bending loss are 0.008 dB and 0.015 dB respectively. The typical effective area of optical fiber at 1550 nm wavelength can reach 130 μm2.