Elastic backscattering spectroscopy is an effective approach to study the particulate composition of biological cells. This paper explores the experimental methods to measure and obtain the elastic backscattering spectra and their advantages and disadvantages. Regarding the microsphere measurement, the pros and cons of two popular spectral acquiring methods are experimentally compared and analyzed, including the method based on a fiber probe and that based on a lens system. The experiments demonstrate that the cross-correlation coefficient between measured spectra by lens system and the Mie calculation is 0.96, higher than 0.93 that is by the fiber probe under an optimum condition, because the lens system results in a well-defined scattering angle for the acquired spectrum. Therefore, it points out when a higher precision in particle sizing is required, the lens system is a better choice and also the filtering denoising will be used to improve the precision of particle sizing. As the spectrum capturing by the optical probe is simple and easy to be implemented, it can be used in a spectral capturing needed by a lower precision.