• Advanced Photonics
  • Vol. 6, Issue 1, 016003 (2024)
Giorgio Tortarolo1,†, Alessandro Zunino1, Simonluca Piazza1,2, Mattia Donato1..., Sabrina Zappone1,3, Agnieszka Pierzyńska-Mach4, Marco Castello1,2,* and Giuseppe Vicidomini1,*|Show fewer author(s)
Author Affiliations
  • 1Istituto Italiano di Tecnologia, Molecular Microscopy and Spectroscopy, Genoa, Italy
  • 2Genoa Instruments, Genoa, Italy
  • 3University of Genoa, Dipartimento di Informatica, Bioingegneria, Robotica e Ingegneria dei Sistemi, Genoa, Italy
  • 4Istituto Italiano di Tecnologia, Nanoscopy and NIC@IIT, Genoa, Italy
  • show less
    DOI: 10.1117/1.AP.6.1.016003 Cite this Article Set citation alerts
    Giorgio Tortarolo, Alessandro Zunino, Simonluca Piazza, Mattia Donato, Sabrina Zappone, Agnieszka Pierzyńska-Mach, Marco Castello, Giuseppe Vicidomini, "Compact and effective photon-resolved image scanning microscope," Adv. Photon. 6, 016003 (2024) Copy Citation Text show less

    Abstract

    Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spatiotemporal information, opening new perspectives for gentle and quantitative superresolution imaging. However, a flawless recording of this information poses significant challenges for the microscope data acquisition (DAQ) system. We present a DAQ module based on the digital frequency domain principle, able to record essential spatial and temporal features of photons. We use this module to extend the capabilities of established imaging techniques based on single-photon avalanche diode (SPAD) array detectors, such as fluorescence lifetime image scanning microscopy. Furthermore, we use the module to introduce a robust multispecies approach encoding the fluorophore excitation spectra in the time domain. Finally, we combine time-resolved stimulated emission depletion microscopy with image scanning microscopy, boosting spatial resolution. Our results demonstrate how a conventional fluorescence laser scanning microscope can transform into a simple, information-rich, superresolved imaging system with the simple addition of a SPAD array detector with a tailored data acquisition system. We expected a blooming of advanced single-photon imaging techniques, which effectively harness all the sample information encoded in each photon.

    Video Introduction to the Article

    视频暂时无法播放,请稍后刷新重试 #007.4
    00:00
    00:00 / 04:13
    弹幕
    vr模式
    超清
    高清
    流畅
    自动
    2x
    1.5x
    1.2x
    1x
    0.5x
    字幕
    模式
    视频音频
    线路
    vr模式
    裸眼眼镜
    播放器
    FlashHTML5
    画中画
    网页全屏
    全屏
    Version:v1.60.2
    • 由酷播云提供服务
    Supplementary Materials
    Giorgio Tortarolo, Alessandro Zunino, Simonluca Piazza, Mattia Donato, Sabrina Zappone, Agnieszka Pierzyńska-Mach, Marco Castello, Giuseppe Vicidomini, "Compact and effective photon-resolved image scanning microscope," Adv. Photon. 6, 016003 (2024)
    Download Citation