A large-scale spatial angle measurement method is proposed based on inertial reference. Common measurement reference is established in inertial space, and the spatial vector coordinates of each measured axis in inertial space are measured by using autocollimation tracking and inertial measurement technology. According to the spatial coordinates of each test vector axis, the measurement of large-scale spatial angle is easily realized. The pointing error of tracking device based on the two mirrors in the measurement system is studied, and the influence of different installation errors to the pointing error is analyzed. This research can lay a foundation for error allocation, calibration and compensation for the measurement system.