Radiometric calibration of the directional polarization camera (DPC) with high-precision is very important for quantitative scientific application of acquiring an accurate description of aerosol distributions and microphysical properties. Using Fourier series analysis method, a polarization rate measurement model is established to eliminate the measurement error introduced by the absolute direction in the angle of linear polarization of the reference light source, so that the polarization property is measured with high accuracy. Combining radiometric and polarimetric calibration model of DPC, radiometric calibration uncertainty caused by polarization property decreased from 8.8% to 2.2% after correction. Using an integrating sphere light source with large aperture and high-precision two-dimensional turntable platform, adopting sectional viewing field measurement method, the entire pixels response non-uniformity is measured and corrected, and the precision of relative radiometric calibration coefficient is better than 0.5%. To transfer the spectral radiance value, the standard lamp and diffuse reflection reference board system is introduced. According to analysis and evaluation of various factors, radiometric calibration accuracy of the entire waveband is better than 5%.