In order to meet the requirements of optical devices for passing infrared waves and shielding electromagnetic waves, a transparent metal mesh shielding film was fabricated on PET flexible substrates. The influence of mesh parameters on shielding efficiency and transmittance was analyzed, and the structural parameters were selected. For the flexibility and thermal stability of PET substrate, high-quality graphics structure was obtained after optimizing pulling speed, baking time, temperature and other parameters in the lithography process. Transparent shielding film was prepared by magnetron sputtering and the film/substrate bonding was strengthened by optimizing sputtering power, sputtering pressure and other parameters. Finally, a metal mesh transparent shielding film with a line width of 3 μm and a cycle of 250 μm was made. The average transmittance in the 300～2 200 nm band was 77% by spectrophotometer, the electromagnetic shielding efficiency in the 2~18 GHz band which measured by shielding room method is higher than 12 dB.