Optical microsphere resonator has the advantages of high quality factor (Q), low threshold, and easy to integrate. In order to make better use of microsphere resonator, high Qlaser can be obtained.In this paper, we successfully prepared perylene doped polystyrene microspheres by solvent-nonsolvent fast injection method.By using this method the diameter of the microspheres can be easily adjusted by changing the parameters of experiments. The microspheres have a smooth surface and intact morphological features, which facilitate whispering gallery modes generation in the sphere cavities.Under the femtosecond laser pumping, we can easily obtain the laser signals from either single microsphere or aggregated microspheres, which shows promising applications.