For the imaging requirements such as high frame rate, global shutter and adjustable output channel amount, a kind of CMOS image sensor for hyperspectral imaging is proposed. The schematic plan and working principles of 8T pixel structure and readout circuit are introduced in detail. Postsimulation and tapeout of this sensor were completed, and the experimental results show the design meets the expectation by achieving targeting features and realizing highspeed and lownoise analog signal output under the condition of large layout area and small amount of output channels. The pixel array size of 2048×256 and the pixel pitch of 24μm×24μm are achieved, the maximum signal output frequency for a single channel is 40×106pixel/s and the frame rate can reach up to 4000f/s, being able to meet the general requirement of a hyperspectral imaging system.