The remarkable and mysterious tonic effect of Ophiocordyceps sinensis has always concerned people. In particular, its pharmaceutical value and total arsenic exceeded the standard, which caused many doubts about Ophiocordyceps sinensis. Therefore, it is necessary to analyze the level of essential trace elements in Ophiocordyceps sinensis and conduct safety evaluations on potentially toxic trace elements. Based on the atomic emission spectrum, atomic absorption spectrum, and atomic fluorescence spectrum, a better analysis scheme is selected according to factors such as applicability, sensitivity, precision, linear range, and the ability to resist the forced interference of major elements with the target analytes. Select a microwave-assisted digestion program to minimize matrix interference. The content of five elements Fe, Zn, Mn, Cu were analyzed by ICP-OES spectrometry, the content of Pb was analyzed by graphite furnace atomic absorption spectrometry, and the content of Se and As was analyzed by tomic fluorescence spectrometry. The average contents of eight trace elements in Ophiocordyceps sinensis from 15 producing areas were analyzed. They are Fe, Zn, Mn, Se, Cu, Sr, As, Pb. The results showed that the average content of Mn, Fe, Zn and Se in Ophiocordyceps sinensis is at a high level, and the average content of Cu and Sr in Ophiocordyceps sinensis is at a general level, the average content of Pb is within the limits of the Pharmacopoeia. The average content of As is beyond the limits of the Pharmacopoeia. The average content of Fe was 1 770.5 μg·g-1, that of Zn was 106.2 μg·g-1, that of Mn was 60.5 μg·g-1, that of Se was 0.055 μg·g-1, that of Cu was 17.4 μg·g-1, that of Sr was 4.4 μg·g-1, that of As was 11.42 μg·g-1, and that of Pb was 2.33 μg·g-1. The average content of Mn and Fe in Ophiocordyceps sinensis from Maqin County, Qinghai province, reached the highest levels of 84.1 and 3 089.8 μg·g-1 , respectively. The average content of Zn in Ophiocordyceps sinensis from Qilian County, Qinghai province, reached the highest level of 163. 0 μg·g-1. The average content of Se in Ophiocordyceps sinensis from Zhiduo County, Qinghai province, reached 0.083 μg·g-1. There were regional differences in the average contents of Fe, Mn, As, Zn in Ophiocordyceps sinensis.