Based on three-dimensional excitation-emission matrix spectroscopy (3D-EEMs), combined with regional fluorescence integration (FRI) and characteristic fluorescence parameters, the spectral characteristics of dissolved organic matter (DOM) in four different types of natural water in Wuhan were studied. Meanwhile, the results of East Lake in Wuhan were compared with relevant data in the recent ten years. The results showed that five fluorescence components were detected, including protein-like fluorescence component(tyrosine-like(C1), tryptophan-like(C2)), fulvic acid-like fluorescent component(C3), soluble microbial metabolite component (C4) and humic acid-like component(C5). From the perspective of fluorescence characteristics, the fluorescence intensity of components C1, C2 and C3 are the highest in the sampling water of lakes with relatively poor water quality, mild to moderate eutrophication and severe pollution. The fluorescence components of DOM in the relatively clean source water, rainwater and the tap water (as the control) were similar. Only C5 components were different; In terms of the source of the DOM, the DOM of water in Wuhan has a significant mixed input feature, with the main terrestrial source and the supplementary endogenous source. The mean terrigenous components of the river, lake, rainwater, source water are 46.2%,56.4%,54.2% and 51.6%, respectively, and the mean proportions of endogenous source components are 12.3%, 10.1%, 10.4% and 9.7%, respectively. The correlation analysis showed that there was a strong correlation between C1, C2 and C3 components(R²>0.98), mainly from terrestrial sources, with homology; C4 and C5 components also have a strong positive correlation(R²>0.73) with total phosphorus (TP) and total organic carbon (TOC), respectively. Compared to the past 10 years data, the terrestrial components (C1, C2, C3) of DOM in Wuhan East Lake have gradually increased, but decreased sharply in 2020, while C4 and C5 components increased significantly.