To verify the virtual hyperspectral technology for acquiring the internal composition and structure information, the virtual hyperspectral system, which took apples as the test materials, was built . First, we detected the virtual hyperspectral system of the normal apples and ill apples. Then, the virtual hyperspectral of apple slices and apple slices with a piece of red optical filter embedded was detected. Finally, the reflection spectrum of normal apple, ill apple, apple slice and slice with a piece of red filter embedded was detected. The results showed that virtual hyperspectral, which can gain more information than reflection spectrum, could be used for detecting the variation of internal composition and structure. It is a strong evidence that this technology can be used in human body in the future. Virtual hpyerspectral is a new path applied to detecting the biological information, and it can be used for the multi-information and cross-information detection simultaneously and systematically. More foundation for quick disease check-up in vivo was expected to be provided by this technology.